ODVP-6E/ODV-E56和p74蛋白的转录、翻译及免疫定位：云杉色卷蛾颗粒体病毒两种高度保守的与病毒粒子相关的包膜蛋白

Transcription, translation, and immunolocalization of ODVP-6E/ODV-E56 and p74 proteins: two highly conserved ODV-associated envelope proteins of Choristoneura fumiferana Granulovirus.

作者信息

Rashidan Kianoush Khajeh, Nassoury Nasha, Giannopoulos Paresa N, Guertin Claude

机构信息

Institut national de la recherche scientifique-Institut Armand-Frappier 531 Blvd des Prairies, H7V 1B7, Laval, Canada.

出版信息

J Biochem Mol Biol. 2005 Jan 31;38(1):65-70. doi: 10.5483/bmbrep.2005.38.1.065.

DOI:10.5483/bmbrep.2005.38.1.065

Abstract

Choristoneura fumiferana granulovirus (ChfuGV) infection results two types of enveloped virions: Occlusion-derived virus (ODV) and budded virus (BV). Structural proteins ODVP-6E/ODV-E56 and p74 are two major conserved ODV-associated proteins that may be involved in the initiation of viral infection cycle in susceptible host insect larvae. This study presents the characterization of ChfuGV odvp-6e/odv-e56 and p74 transcription and translation as well as immunolocalization of these proteins in the occluded ChfuGV virion. Our results revealed that the transcription of odvp-6e/odv-e56 and p74 genes, both, start at 24 hours post infection (h p.i.). Using monospecific polyclonal antibodies made against ODVP-6E/ODV-E56 and p74 we demonstrated that these proteins are both expressed late in infection (24 h p.i.). Immunogold labeling using antisera against ODVP-6E/ODV-E56 and p74 proteins demonstrated that ODVP-6E/ODV-E56 and p74 proteins are both associated with the ODV envelop of ChfuGV.

摘要

云杉卷叶蛾颗粒体病毒（ChfuGV）感染会产生两种有包膜的病毒粒子：多角体衍生病毒（ODV）和出芽病毒（BV）。结构蛋白ODVP-6E/ODV-E56和p74是两种主要的保守ODV相关蛋白，可能参与易感宿主昆虫幼虫中病毒感染周期的起始。本研究介绍了ChfuGV的odvp-6e/odv-e56和p74转录与翻译的特征，以及这些蛋白在被包埋的ChfuGV病毒粒子中的免疫定位。我们的结果显示，odvp-6e/odv-e56和p74基因的转录均在感染后24小时（h p.i.）开始。使用针对ODVP-6E/ODV-E56和p74制备的单特异性多克隆抗体，我们证明这些蛋白均在感染后期（24 h p.i.）表达。使用针对ODVP-6E/ODV-E56和p74蛋白的抗血清进行免疫金标记，结果表明ODVP-6E/ODV-E56和p74蛋白均与ChfuGV的ODV包膜相关。