Kurose Kouichi, Tohkin Masahiro, Hasegawa Ryuichi
Division of Medicinal Safety Science, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Biochim Biophys Acta. 2005 Feb 14;1727(2):141-4. doi: 10.1016/j.bbaexp.2004.12.003. Epub 2004 Dec 30.
PREX is a positive regulatory element for xenobiotic responsive element (XRE)-mediated gene expression that is located upstream of the XRE in the CYP2A8 gene. Using gel mobility shift assays, we demonstrated that NF2d9 (LBP-1a), a transcription factor related to CP2 (LBP-1c/LSF), bound directly to PREX and also interacts indirectly with XRE. Luciferase-reporter gene assays showed that the overexpression of NF2d9 enhanced PREX and XRE-driven CYP2A8 gene transcriptional induction. These findings suggest that the interaction of NF2d9 with PREX and XRE enhances XRE-driven CYP2A8 gene transcriptional induction.
PREX是一种位于CYP2A8基因中外源化合物反应元件（XRE）上游的、介导XRE基因表达的正向调控元件。通过凝胶迁移率变动分析，我们证明了与CP2（LBP-1c/LSF）相关的转录因子NF2d9（LBP-1a）直接与PREX结合，并且还间接与XRE相互作用。荧光素酶报告基因分析表明，NF2d9的过表达增强了PREX和XRE驱动的CYP2A8基因转录诱导。这些发现表明，NF2d9与PREX和XRE的相互作用增强了XRE驱动的CYP2A8基因转录诱导。
