从光肩星天牛（Acalolepta luxuriosa）中纯化并克隆具有Kunitz结构域的新型抗菌肽luxuriosin的cDNA

Purification and cDNA cloning of luxuriosin, a novel antibacterial peptide with Kunitz domain from the longicorn beetle, Acalolepta luxuriosa.

作者信息

Ueda Kenjiro, Saito Ayaka, Imamura Morikazu, Miura Nami, Atsumi Shogo, Tabunoki Hiroko, Watanabe Ayako, Kitami Madoka, Sato Ryoichi

机构信息

Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Koganei, Tokyo 184-8588, Japan.

出版信息

Biochim Biophys Acta. 2005 Feb 11;1722(1):36-42. doi: 10.1016/j.bbagen.2004.11.014. Epub 2004 Dec 19.

DOI:10.1016/j.bbagen.2004.11.014

PMID:15716136

Abstract

We have purified a novel antibacterial peptide from the hemolymph of the coleopteran insect Acalolepta luxuriosa, of the family Cerambyocidae, and named it luxuriosin. This peptide showed growth-inhibitory activity against Micrococcus luteus and germination- and/or growth-inhibitory activity against the conidia from rice blast fungus, Magnaporthe grisea. The amino acid sequence determined by cDNA cloning identified luxuriosin as a peptide of 88 amino acids with a theoretical molecular weight of 10,368.34, containing a Kunitz domain.

摘要

我们从天牛科昆虫华丽柄天牛（Acalolepta luxuriosa）的血淋巴中纯化出一种新型抗菌肽，并将其命名为华丽菌素（luxuriosin）。该肽对藤黄微球菌显示出生长抑制活性，对稻瘟病菌（Magnaporthe grisea）的分生孢子具有萌发和/或生长抑制活性。通过cDNA克隆确定的氨基酸序列表明，华丽菌素是一种由88个氨基酸组成的肽，理论分子量为10368.34，含有一个库尼兹结构域。