Davidson Ben, Xi Zhijun, Klokk Tove Irene, Tropé Claes G, Dørum Anne, Scheistrøen Marit, Saatcioglu Fahri
Department of Pathology, the Norwegian Radium Hospital, Oslo, Norway.
Am J Clin Pathol. 2005 Mar;123(3):360-8. doi: 10.1309/PTBB-5BPC-KX8K-9V69.
We immunohistochemically analyzed kallikrein 4 protein (hK4) expression in patients with epithelial ovarian carcinoma (181 malignant effusions and 103 solid carcinoma lesions). Expression of hK4 was also studied in 32 effusions using immunoblotting. Carcinoma cells expressed hK4 in 144 (79.6%) of 181 effusions and 85 (82.5%) of 103 solid tumors. Expression was seen in 51% or more of tumor cells in 70 effusions but often was limited to 5% or fewer cells in solid tumors (P = .009, primary tumors vs effusions; P = .002, metastases vs effusions). Immunoblotting showed hK4 expression in 31 of 32 specimens. Stromal cell hK4 expression, seen in 48 (46.6%) of 103 lesions, was significantly higher in primary tumors than metastases (26/43 vs 22/60, P = .019). hK4 expression in tumor cells was significantly lower in International Federation of Gynecology and Obstetrics stage IV than stage III tumors (P = .004, all lesions; P = .012, primary tumors). hK4 expression in carcinoma cells was associated with longer overall survival (not significant; P = .14, peritoneal effusions). hK4 is expressed widely in ovarian carcinoma; levels in carcinoma cells are highest in effusions, which might be related to loss of stromal contribution and/or altered microenvironment. hK4 expression in carcinoma cells of effusions or solid tumors does not predict survival.
我们采用免疫组化方法分析了上皮性卵巢癌患者（181例恶性积液和103例实体癌病灶）中激肽释放酶4蛋白（hK4）的表达情况。还采用免疫印迹法研究了32例积液中hK4的表达。在181例积液中的144例（79.6%）和103例实体瘤中的85例（82.5%）中，癌细胞表达hK4。在70例积液中，51%或更多的肿瘤细胞有表达，但在实体瘤中表达通常限于5%或更少的细胞（原发性肿瘤与积液相比，P = 0.009；转移瘤与积液相比，P = 0.002）。免疫印迹显示32例标本中有31例表达hK4。在103个病灶中的48个（46.6%）可见基质细胞hK4表达，原发性肿瘤中的表达明显高于转移瘤（26/43比22/60，P = 0.019）。国际妇产科联盟IV期肿瘤细胞中的hK4表达明显低于III期肿瘤（所有病灶，P = 0.004；原发性肿瘤，P = 0.012）。癌细胞中hK4表达与总生存期延长相关（无显著性差异；P = 0.14，腹膜积液）。hK4在卵巢癌中广泛表达；癌细胞中的水平在积液中最高，这可能与基质贡献的丧失和/或微环境改变有关。积液或实体瘤癌细胞中的hK4表达不能预测生存期。
