Marcol Wiesław, Kotulska Katarzyna, Larysz-Brysz Magdalena, Pietrucha-Dutczak Marita, Olakowska Edyta, Malinowska Izabela, Lewin-Kowalik Joanna
Department of Physiology, Medical University of Silesia, Katowice, Poland.
Ital J Anat Embryol. 2004 Oct-Dec;109(4):199-208.
Transected peripheral nerve can be protected with different supplementations. One of them is implantation of dead-ended connective tissue chambers filled with fibrin and growth-promoting substances. The aim of this study was to find whether nerve growth factor (NGF) applied by means of such method exerts neuroprotective effect upon transected sciatic nerves. Study was performed on the adult male Wistar C rats. Connective tissue chambers grew around the silicone tubes implanted under their skin. Chambers were then filled with fibrin (control group) or fibrin with NGF solution (NGF group). Right sciatic nerve was cut, its distal stump was removed and its proximal stump was introduced into the chamber. Following 4 weeks DiI was applied to the free end of implant. The labeled motoneurons in the slices obtained from L3-L4 spinal cord segments and the number of myelinated nerve fibers present in the middle part of the chambers were counted. Acetylcholinesterase-positive fiber endings inside the chambers were also visualized. Our data showed that the number of motor neurons and their diameters as well as the number of myelinated fibers were higher in the NGF group when compared to the control group, but these differences were not significant. In both groups parallelly arranged acetylcholinesterase-positive nerve fibers were present. The obtained results show that NGF has no influence on regeneration of the motor component of the rat sciatic nerves in adult animals.
横断的周围神经可以通过不同的补充方法来保护。其中一种方法是植入充满纤维蛋白和促生长物质的盲端结缔组织腔室。本研究的目的是探究通过这种方法应用神经生长因子（NGF）是否对横断的坐骨神经发挥神经保护作用。研究在成年雄性Wistar C大鼠身上进行。结缔组织腔室在植入其皮下的硅胶管周围生长。然后腔室分别填充纤维蛋白（对照组）或含NGF溶液的纤维蛋白（NGF组）。切断右侧坐骨神经，去除其远端残端，并将其近端残端引入腔室。4周后，将碘化丙啶（DiI）应用于植入物的自由端。对从L3-L4脊髓节段获得的切片中标记的运动神经元以及腔室中部存在的有髓神经纤维数量进行计数。还观察了腔室内乙酰胆碱酯酶阳性纤维末梢。我们的数据显示，与对照组相比，NGF组的运动神经元数量及其直径以及有髓纤维数量更高，但这些差异并不显著。两组均存在平行排列的乙酰胆碱酯酶阳性神经纤维。所得结果表明，在成年动物中，NGF对大鼠坐骨神经运动成分的再生没有影响。
