Xing James Zan, Zhu Lijun, Jackson Jo Ann, Gabos Stephan, Sun Xue-Jun, Wang Xiao-Bo, Xu Xiao
Department of Laboratory Medicine and Pathology, University of Alberta, Health Surveillance, Edmonton, Alberta, Canada.
Chem Res Toxicol. 2005 Feb;18(2):154-61. doi: 10.1021/tx049721s.
A real-time cell electronic sensing (RT-CES) system was used for label-free, dynamic measurement of cell responses to cytotoxicants. Cells were grown onto the surfaces of microelectronic sensors, which are comprised of circle-on-line electrode arrays and are integrated into the bottom surfaces of the microtiter plate. Changes in cell status such as cell number, viability, morphology, and adherence were monitored and quantified by detecting sensor electrical impedance. For cell quantification and viability measurement, the data generated on the RT-CES system correlated well with those from the colorimetric (MTT) assay. For cytotoxicity assessment, cells growing on microelectronic sensors were treated with different cytotoxicants, such as arsenic, mercury, and sodium dichromate. The dynamic responses of the cells to the toxicants were continuously monitored by the RT-CES system. On the basis of the IC50 values, the RT-CES system displays an equal sensitivity to the neutral red uptake assay at specific time points. Furthermore, because the RT-CES system provides real-time information regarding the state of cell morphology and adhesion in addition to cell number, we were able to discern a previously unreported effect of arsenic on NIH 3T3 cells prior to cell death. Also, using the RT-CES system, we were able to monitor cytotoxicity effects that occur within a minute of compound addition. Taken together, the RT-CES system allows for real-time, continuous monitoring and quantitative recording of the whole assay process and provides new insight into the cell-toxicant interaction.
实时细胞电子传感（RT-CES）系统用于对细胞对细胞毒性剂的反应进行无标记动态测量。将细胞培养在微电子传感器表面，该传感器由在线圆形电极阵列组成，并集成在微量滴定板的底面。通过检测传感器电阻抗来监测和量化细胞状态的变化，如细胞数量、活力、形态和粘附情况。对于细胞定量和活力测量，RT-CES系统生成的数据与比色法（MTT）测定的数据相关性良好。对于细胞毒性评估，将生长在微电子传感器上的细胞用不同的细胞毒性剂处理，如砷、汞和重铬酸钠。RT-CES系统持续监测细胞对这些毒物的动态反应。基于IC50值，RT-CES系统在特定时间点对中性红摄取试验显示出同等的敏感性。此外，由于RT-CES系统除了提供细胞数量信息外，还能提供有关细胞形态和粘附状态的实时信息，我们能够在细胞死亡之前识别出砷对NIH 3T3细胞的一种以前未报道的作用。而且，使用RT-CES系统，我们能够监测化合物添加后一分钟内发生的细胞毒性作用。综上所述，RT-CES系统能够对整个检测过程进行实时、连续监测和定量记录，并为细胞与毒物的相互作用提供了新的见解。
