Possible role of NF-kappaB in Bcl-X(L) protection against hydrogen peroxide-induced PC12 cell death.

Bcl-X(L), a mitochondrial membrane protein, blocks apoptosis induced by a wide array of death signals. In spite of extensive research, the molecular milieu that characterizes the anti-apoptotic function of Bcl-X(L) is complex and not fully clarified. In the present work, we have investigated the role of Bcl-X(L) in protecting against oxidative death induced by H(2)O(2) in cultured rat pheochromocytoma (PC12) cells. PC12 cells exposed to H(2)O(2) underwent apoptotic cell death as determined by internucleosomal DNA fragmentation and an increased pro-apoptotic Bax to anti-apoptotic Bcl-X(L) ratio. Moreover, stable transfection with the bcl-X(L) gene rescued PC12 cells from apoptotic death caused by H(2)O(2). PC12 cells overexpressing bcl-X(L) exhibited relatively high constitutive transcriptional as well as DNA binding activities of NF-kappaB, compared with the vector-transfected control cells. Addition of NF-kappaB inhibitors, such as parthenolide and N-tosyl-L-phenylalanine chloromethyl ketone, to the media aggravated H(2)O(2)-induced oxidative cell death. PC12 cells transfected with bcl-X(L) exhibited higher levels of the heme oxygenase-1, which may confer these cells protection against oxidative stress. These results suggest that the redox-sensitive transcription factor NF-kappaB may play a role in bcl-X(L)-mediated protection against oxidative cell death.