Vaessen L M, Baan C C, Ouwehand A J, Jutte N H, Balk A H, Mochtar B, Claas F H, Weimar W
Department of Internal Medicine I, University Hospital Dijkzigt-Rotterdam, The Netherlands.
Clin Exp Immunol. 1992 May;88(2):213-9. doi: 10.1111/j.1365-2249.1992.tb03064.x.
In vivo-activated, committed donor-specific cytotoxic lymphocytes (cCTL) can be propagated and expanded from endomyocardial biopsies (EMB) in IL-2-enriched medium especially during an acute rejection episode. We report here our efforts to detect these cCTL by the same technique in peripheral blood at the moment of rejection and when no rejection was diagnosed. During or just before rejection, significantly less frequent (P less than 0.01) donor reactive cCTL were found in PBL samples (two out of 20) than in the simultaneously taken EMB samples (13 out of 19). Donor B-LCL and/or third-party B-LCL were lysed by 15 PBL samples. Inhibition studies revealed that this lysis was due to LAK-like cytotoxicity. The results show that peripheral blood does not reflect intra-graft events, which is probably the reason for the irreproducible results of diagnosis of rejection by monitoring immunological parameters in the peripheral blood.
体内激活的、定向的供体特异性细胞毒性淋巴细胞（cCTL），尤其是在急性排斥反应期间，可在富含白细胞介素-2的培养基中从心内膜心肌活检（EMB）中增殖和扩增。我们在此报告我们通过相同技术在排斥反应发生时以及未诊断出排斥反应时在外周血中检测这些cCTL的努力。在排斥反应期间或即将发生排斥反应之前，在外周血淋巴细胞（PBL）样本（20例中有2例）中发现的供体反应性cCTL频率明显低于同时采集的EMB样本（19例中有13例）（P小于0.01）。15个PBL样本可裂解供体B淋巴母细胞系（B-LCL）和/或第三方B-LCL。抑制研究表明，这种裂解是由于类似淋巴因子激活的杀伤细胞（LAK）的细胞毒性。结果表明，外周血不能反映移植物内的事件，这可能是通过监测外周血免疫参数诊断排斥反应结果不可重复的原因。
