Evans-Nguyen Kenyon M, Schoenfisch Mark H
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
Langmuir. 2005 Mar 1;21(5):1691-4. doi: 10.1021/la047303h.
Fibrin proliferation from both human fibrinogen solutions and platelet-poor plasma was studied quantitatively as a function of substrate surface properties. A quartz crystal microbalance was used to monitor both protein adsorption and fibrin proliferation in real time at hydrophobic, hydrophilic, positively charged, and negatively charged surfaces. Scanning electron microscopy was used to characterize the morphology of the polymerized fibrin layers. The observed changes in mass indicate that fibrinogen adsorption occurs rapidly and mediates subsequent fibrin proliferation. Notably, substrate surface properties significantly affect the ability of adsorbed fibrinogen to promote fibrin proliferation.
研究了来自人纤维蛋白原溶液和乏血小板血浆的纤维蛋白增殖与底物表面性质之间的定量关系。使用石英晶体微天平实时监测疏水、亲水、带正电和带负电表面上的蛋白质吸附和纤维蛋白增殖情况。采用扫描电子显微镜对聚合纤维蛋白层的形态进行表征。观察到的质量变化表明纤维蛋白原吸附迅速并介导随后的纤维蛋白增殖。值得注意的是，底物表面性质显著影响吸附的纤维蛋白原促进纤维蛋白增殖的能力。
