黄连素对3T3-L1脂肪细胞脂联素mRNA表达的影响
[Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte].
作者信息
Gu Wei, Zeng Wen-heng, Hu Hai-ying
机构信息
The 2nd Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310009, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2005 Feb;30(4):286-8.
OBJECTIVE
To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte.
METHOD
The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours, the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using beta-actin as internal reference.
RESULT
The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only (P < 0.05), the effect of berberine was inhibited by high concentration insulin (P < 0.05).
CONCLUSION
In vitro, berberine increases the expression of adiponectin in 3T3-L1 adipocyte, insulin inhibits the effect of berberine.
目的
探讨黄连素和胰岛素对3T3-L1脂肪细胞脂联素mRNA表达的影响。
方法
用黄连素和胰岛素处理3T3-L1脂肪细胞48小时，以β-肌动蛋白为内参，采用半定量RT-PCR法测定3T3-L1脂肪细胞中脂联素mRNA的表达水平。
结果
仅用黄连素处理后，3T3-L1脂肪细胞中脂联素mRNA水平升高（P<0.05），高浓度胰岛素可抑制黄连素的作用（P<0.05）。
结论
在体外，黄连素可增加3T3-L1脂肪细胞中脂联素的表达，胰岛素可抑制黄连素的作用。
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