He Yan, Francis Franto, Myers Kenneth A, Yu Wenqian, Black Mark M, Baas Peter W
Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USA.
J Cell Biol. 2005 Feb 28;168(5):697-703. doi: 10.1083/jcb.200407191. Epub 2005 Feb 22.
Recent studies have shown that the transport of microtubules (MTs) and neurofilaments (NFs) within the axon is rapid, infrequent, asynchronous, and bidirectional. Here, we used RNA interference to investigate the role of cytoplasmic dynein in powering these transport events. To reveal transport of MTs and NFs, we expressed EGFP-tagged tubulin or NF proteins in cultured rat sympathetic neurons and performed live-cell imaging of the fluorescent cytoskeletal elements in photobleached regions of the axon. The occurrence of anterograde MT and retrograde NF movements was significantly diminished in neurons that had been depleted of dynein heavy chain, whereas the occurrence of retrograde MT and anterograde NF movements was unaffected. These results support a cargo model for NF transport and a sliding filament model for MT transport.
最近的研究表明，微管（MTs）和神经丝（NFs）在轴突内的运输是快速、不频繁、异步且双向的。在此，我们使用RNA干扰来研究胞质动力蛋白在驱动这些运输事件中的作用。为了揭示MTs和NFs的运输，我们在培养的大鼠交感神经元中表达了EGFP标记的微管蛋白或NF蛋白，并对轴突光漂白区域的荧光细胞骨架元件进行活细胞成像。在动力蛋白重链缺失的神经元中，顺行MT和逆行NF运动的发生率显著降低，而逆行MT和顺行NF运动的发生率则不受影响。这些结果支持了NF运输的货物模型和MT运输的滑动丝模型。
