Canadien Veronica, Tan Tracy, Zilber Rachelle, Szeto Jason, Perrin Andrew J, Brumell John H
Infection, Immunity, Injury, and Repair Program, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.
J Immunol. 2005 Mar 1;174(5):2471-5. doi: 10.4049/jimmunol.174.5.2471.
In response to a maturation stimulus, dendritic cells undergo the formation of ubiquitinated protein aggregates known as dendritic cell aggresome-like induced structures (DALIS). DALIS are thought to act as Ag storage structures, allowing for the prioritized degradation of proteins during infection. In this study, we demonstrate that murine macrophages can also form ubiquitinated protein aggregates that are indistinguishable from DALIS. These were formed in a dose- and time-dependent manner, and in response to a variety of microbial products. Surprisingly, the proteasome did not accumulate on these ubiquitinated protein structures, further underlining the difference between DALIS and aggresomes. Our studies suggest that DALIS formation is important for the function of Ag-presenting immune cells during infection.
在对成熟刺激的反应中，树突状细胞会形成被称为树突状细胞聚集体样诱导结构（DALIS）的泛素化蛋白聚集体。DALIS被认为作为抗原储存结构，在感染期间允许蛋白质优先降解。在本研究中，我们证明小鼠巨噬细胞也能形成与DALIS无法区分的泛素化蛋白聚集体。这些聚集体以剂量和时间依赖性方式形成，并对多种微生物产物产生反应。令人惊讶的是，蛋白酶体并未在这些泛素化蛋白结构上积累，这进一步突显了DALIS与聚集体之间的差异。我们的研究表明，DALIS的形成对于感染期间抗原呈递免疫细胞的功能很重要。
