Kirik Victor, Lee Myeong Min, Wester Katja, Herrmann Ullrich, Zheng Zhengui, Oppenheimer David, Schiefelbein John, Hulskamp Martin
Botanical Institute, University of Cologne, Gyrhofstrasse 15, 50931 Cologne, Germany.
Development. 2005 Apr;132(7):1477-85. doi: 10.1242/dev.01708. Epub 2005 Feb 23.
The functional diversification of duplicated genes is one of the driving forces in evolution. To understand the molecular mechanisms of gene diversification, we studied the functional relationship of the two Arabidopsis paralogous MYB-related genes GL1 and MYB23. We show that MYB23 controls trichome branching and trichome initiation at leaf edges. The latter is controlled redundantly together with GL1. We show that the two proteins are functionally equivalent during trichome initiation but not during trichome branching. RT-PCR and reporter construct analysis revealed spatial, temporal and genetic differences in transcriptional regulation of the GL1 and MYB23 genes. Presented data indicate that the diversification of GL1 and MYB23 gene functions occurred at the level of cis-regulatory sequences with respect to trichome initiation, and that, in parallel, the diversification with respect to regulation of trichome branching also involved changes in respective proteins.
基因复制后的功能分化是进化的驱动力之一。为了解基因分化的分子机制，我们研究了拟南芥中两个同源的MYB相关基因GL1和MYB23的功能关系。我们发现MYB23控制叶边缘的毛状体分支和毛状体起始。后者与GL1一起发挥冗余控制作用。我们发现这两种蛋白质在毛状体起始过程中功能等同，但在毛状体分支过程中并非如此。RT-PCR和报告基因构建体分析揭示了GL1和MYB23基因转录调控在空间、时间和遗传上的差异。现有数据表明，GL1和MYB23基因功能的分化发生在毛状体起始相关的顺式调控序列水平上，并且与此同时，毛状体分支调控方面的分化还涉及各自蛋白质的变化。
