Yamasaki Katsutoshi, Komatsu Masaru, Shimakawa Koichi, Satoh Kaori, Nishio Hisaaki, Sueyoshi Noriyuki, Toyokawa Masahiro, Sakamoto Masako, Higuchi Takeshi, Wada Yasunao, Kofuku Tomomi, Orita Tamaki, Yamashita Tomonari, Kinoshita Shohiro, Aihara Masanori
Clinical Laboratory, Wakayama Rosai Hospital, 435 Koya, Wakayama, 640-8345, Japan.
J Infect Chemother. 2005 Feb;11(1):9-13. doi: 10.1007/s10156-004-0352-0.
We studied the antimicrobial susceptibility of AmpC beta-lactamase-producing Escherichia coli isolates collected at ten medical institutions in the Kinki area of Japan during a 6-month period (November 2002 through April 2003). Of 2845 E. coli isolates tested, 29 (1.0%) showed a minimum inhibitory concentration (MIC) for cefazolin of more than 8 microg/ml and were three-dimensional extract test positive. In standard inoculum susceptibility tests against these 29 strains, the MIC90s for the four carbapenems tested ranged from 0.06 microg/ml to 0.5 microg/ml, and these compounds were more active than the other beta-lactams, with meropenem being the most active. The MIC90s for beta-lactams, except carbapenems, ranged from 4 microg/ml to 32 microg/ml, with cefepime being the most active. In high inoculum susceptibility tests against these strains, the MIC90s for the four carbapenems and cefepime were 8 microg/ml or less, and these compounds were more active than other beta-lactams. The MIC90s for beta-lactams, except carbapenems and cefepime, were 32 microg/ml or more. The MIC90s for the five quinolones tested ranged from 4 microg/ml to 16 microg/ml, and the order of increasing susceptibility was ciprofloxacin > levofloxacin, gatifloxacin and pazufloxacin > prulifloxacin.
我们研究了2002年11月至2003年4月这6个月期间，从日本近畿地区10家医疗机构收集的产AmpCβ-内酰胺酶大肠埃希菌分离株的抗菌药敏情况。在检测的2845株大肠埃希菌分离株中，29株（1.0%）对头孢唑林的最低抑菌浓度（MIC）大于8μg/ml，三维提取试验呈阳性。在针对这29株菌的标准接种量药敏试验中，所检测的4种碳青霉烯类药物的MIC90范围为0.06μg/ml至0.5μg/ml，这些化合物比其他β-内酰胺类药物活性更强，其中美罗培南活性最强。除碳青霉烯类药物外，其他β-内酰胺类药物的MIC90范围为4μg/ml至32μg/ml，头孢吡肟活性最强。在针对这些菌株的高接种量药敏试验中，4种碳青霉烯类药物和头孢吡肟的MIC90为8μg/ml或更低，这些化合物比其他β-内酰胺类药物活性更强。除碳青霉烯类药物和头孢吡肟外，其他β-内酰胺类药物的MIC90为32μg/ml或更高。所检测的5种喹诺酮类药物的MIC90范围为4μg/ml至16μg/ml，敏感性增加顺序为环丙沙星>左氧氟沙星、加替沙星和帕珠沙星>普卢利沙星。
