Ding Zuoqi, Dai Yue, Wang Zhengtao
Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, People's Republic of China.
Planta Med. 2005 Feb;71(2):183-5. doi: 10.1055/s-2005-837789.
Scopoletin exhibited an immediate and dose-dependent hypouricemic effect after intraperitoneal administration (50, 100, 200 mg/kg) in hyperuricemic mice induced by potassium oxonate; however, it did not affect the serum uric acid level in normal mice at the tested doses. For exploring the involved mechanisms of action of scopoletin, potential inhibitory effects on xanthine oxidase and possible uricosuric effects were investigated. Scopoletin (50, 100, 200 mg/kg) significantly inhibited the activity of xanthine oxidase in liver homogenates of hyperuricemic mice although it only showed a relatively weak, albeit competitive-type, inhibition of xanthine oxidase in a commercial assay. Furthermore, a potent uricosuric effect of scopoletin (100, 200 mg/kg) was ascertained. These results demonstrated for the first time that scopoletin exhibits, hypouricemic activities through decreasing uric acid production and as well as a uricosuric mechanism.
在草酸钾诱导的高尿酸血症小鼠中，腹腔注射东莨菪亭（50、100、200mg/kg）后呈现出即时且剂量依赖性的降尿酸作用；然而，在测试剂量下，它对正常小鼠的血清尿酸水平没有影响。为了探究东莨菪亭的作用机制，研究了其对黄嘌呤氧化酶的潜在抑制作用以及可能的促尿酸排泄作用。东莨菪亭（50、100、200mg/kg）显著抑制高尿酸血症小鼠肝脏匀浆中黄嘌呤氧化酶的活性，尽管在商业检测中它仅表现出相对较弱的（尽管是竞争性类型的）对黄嘌呤氧化酶的抑制作用。此外，确定了东莨菪亭（100、200mg/kg）具有显著的促尿酸排泄作用。这些结果首次证明，东莨菪亭通过减少尿酸生成以及促尿酸排泄机制发挥降尿酸活性。
