Nolan Matthew J, Cribb Thomas H
Department of Microbiology and Parasitology and the Centre for Marine Studies, The University of Queensland, Brisbane, Queensland 4072, Australia.
Folia Parasitol (Praha). 2004 Dec;51(4):320-6. doi: 10.14411/fp.2004.040.
The sanguinicolids Paracardicoloides yamagutii Martin, 1974 and Plethorchis acanthus Martin, 1975 were obtained from their definitive hosts, Anguilla reinhardtii Steindachner and Mugil cephalus Linnaeus (respectively) in the tributaries of the Brisbane River, Queensland, Australia. Two putative sanguinicolid cercariae were collected from a hydrobiid gastropod, Posticobia brazieri Smith, in the same waters. The two cercariae differ markedly in size and the form of their sporocysts. Both putative cercariae develop in the digestive gland of Po. brazieri. The ITS2 rDNA region from these sanguinicolids and a Clinostomum species (utilised as an outgroup due to the close morphological similarities between the cercarial stages of the Clinostomidae and the Sanguinicolidae) were sequenced and aligned. Comparison of the ITS2 sequences showed one cercaria to be that of P. yamagutii. This is the first sanguinicolid life history determined by a molecular method. P. yamagutii is the fourth sanguinicolid known to utilise a freshwater hydrobiid gastropod as its intermediate host. ITS2 rDNA is effective in distinguishing sanguinicolids at the species level.
血居吸虫类的山古氏副心居吸虫（Paracardicoloides yamagutii Martin，1974年）和棘血居吸虫（Plethorchis acanthus Martin，1975年）分别从其终末宿主澳大利亚昆士兰州布里斯班河支流中的新西兰鳗鲡（Anguilla reinhardtii Steindachner）和鲻鱼（Mugil cephalus Linnaeus）体内获得。在同一水域的一种椎实螺科腹足类动物——布氏后沼螺（Posticobia brazieri Smith）体内采集到了两种疑似血居吸虫尾蚴。这两种尾蚴在大小和孢蚴形态上有显著差异。两种疑似尾蚴均在布氏后沼螺的消化腺中发育。对这些血居吸虫类以及一种斜睾吸虫属物种（由于斜睾科和血居科尾蚴阶段在形态上有密切相似性，将其用作外类群）的ITS2核糖体DNA区域进行了测序和比对。ITS2序列比较显示其中一种尾蚴是山古氏副心居吸虫的。这是首次通过分子方法确定的血居吸虫生活史。山古氏副心居吸虫是已知的第四种以淡水椎实螺科腹足类动物作为中间宿主的血居吸虫。ITS2核糖体DNA在物种水平上能有效区分血居吸虫类。
