Wang Qiang, Peng Yi-zhi
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing, 400038, P.R. China.
Zhonghua Shao Shang Za Zhi. 2004 Dec;20(6):327-9.
To investigate the influence of maturative agents, including lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF-alpha) and interferon gamma(IFN-gamma) on the maturation of immature dendritic cells originated from murine bone marrow induced by low dose of granulocyte macrophage colony stimulating factor (rm GM-CSF).
Dendritic cells from murine bone marrow progenitors were cultured in low and high doses of GM-CSF for 6 days, and then the suspending cells were harvested for the experiment. After 3 days of co-culture of the obtained DC with low dose rmGM-CSF (GM(low)DC) with LPS, TNF-alpha and IFN-gamma, the stimulatory capacity of inducing proliferation of non-sensitized splenocytes of GM(low)DC in mixed lymphocyte reaction (MLR) was observed and compared with that of GM(high)DC.
GM(low)DC could not activate the non-sensitized splenocytes or induce it into proliferation after 3 days of co-incubation with LPS, TNF-alpha, IFN-gamma, with the stimulation index (SI) lower than 2. Whereas GM(high)DC could strongly activate naive splenocytes (SI = 4.71).
GM(low)DC was resistant to maturation and insensitive to the stimulation by LPS, TNF-alpha or IFN-gamma.
研究包括脂多糖（LPS）、肿瘤坏死因子α（TNF-α）和干扰素γ（IFN-γ）在内的成熟因子对低剂量粒细胞巨噬细胞集落刺激因子（rm GM-CSF）诱导的源自小鼠骨髓的未成熟树突状细胞成熟的影响。
将小鼠骨髓祖细胞来源的树突状细胞分别用低剂量和高剂量的GM-CSF培养6天，然后收集悬浮细胞用于实验。将获得的DC与低剂量rmGM-CSF（GM(低)DC）与LPS、TNF-α和IFN-γ共培养3天后，观察GM(低)DC在混合淋巴细胞反应（MLR）中诱导未致敏脾细胞增殖的刺激能力，并与GM(高)DC进行比较。
GM(低)DC与LPS、TNF-α、IFN-γ共孵育3天后，不能激活未致敏脾细胞或诱导其增殖，刺激指数（SI）低于2。而GM(高)DC能强烈激活幼稚脾细胞（SI = 4.71）。
GM(低)DC对成熟具有抗性，对LPS、TNF-α或IFN-γ的刺激不敏感。
