Zheng Yi-zhou, Zhang Li, Wang Hui-jun, Han Zhong-chao, Takahashi Tsuneo A
State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300020, China.
Zhonghua Xue Ye Xue Za Zhi. 2004 Dec;25(12):736-9.
To compare the expression profiles of a set of homing-related molecules (HRM) repertoire expressed on hematopoietic stem/progenitor cells (HS/PC) from different sources.
The expression levels of HRM on HS/PC from umbilical cord blood (UCB), mobilized peripheral blood (mPB) and bone marrow (BM) were assessed using a highly sensitive 4-color flow cytometric analysis.
UCB-derived CD34(bright) cells, as well as mPB- and BM-derived CD34(bright) cells strongly expressed CD44, CD11a, CD18, CD62L, CD31 and CD49d. On the other hand, significantly lower expressions of CD49e, CD49f, CXCR-4 and CD54 on UCB-derived CD34(bright) and CD34(bright)CD38(-) cells, compared with those on mPB- and BM-derived CD34(bright) and CD34(bright)CD38(-) cells, were observed. None of UCB-, mPB- and BM-derived CD34(bright) cells expressed other chemokine receptors, including CCR-1, CCR-2, CCR-3, CCR-5, CXCR-1, CXCR-2, CXCR-3 and CXCR-5. Another striking finding was that only mPB-derived CD34(bright) cells expressed significant levels of both the matrix metalloproteinases MMP-2 [(11.4 +/- 4.9)%] and MMP-9 [(27.6 +/- 7.8)%].
HS/PC from UCB have some defects of expression of HRM repertoire, which might partly explain the cause(s) of delayed hematopoietic reconstitution after UCB transplant.
比较不同来源造血干/祖细胞（HS/PC）上一组归巢相关分子（HRM）库的表达谱。
采用高灵敏度四色流式细胞术分析评估脐血（UCB）、动员外周血（mPB）和骨髓（BM）来源的HS/PC上HRM的表达水平。
UCB来源的CD34（明亮）细胞以及mPB和BM来源的CD34（明亮）细胞均强烈表达CD44、CD11a、CD18、CD62L、CD31和CD49d。另一方面，与mPB和BM来源的CD34（明亮）及CD34（明亮）CD38（-）细胞相比，观察到UCB来源的CD34（明亮）和CD34（明亮）CD38（-）细胞上CD49e、CD49f、CXCR - 4和CD54的表达显著降低。UCB、mPB和BM来源的CD34（明亮）细胞均未表达其他趋化因子受体，包括CCR - 1、CCR - 2、CCR - 3、CCR - 5、CXCR - 1、CXCR - 2、CXCR - 3和CXCR - 5。另一个显著发现是只有mPB来源的CD34（明亮）细胞表达显著水平的基质金属蛋白酶MMP - 2[（11.4±4.9）%]和MMP - 9[（27.6±7.8）%]。
UCB来源的HS/PC在HRM库表达方面存在一些缺陷，这可能部分解释了UCB移植后造血重建延迟的原因。
