Zhang Hong-wei, Qiu Zhi-feng, Li Tai-sheng
Department of Infectious Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Yi Xue Za Zhi. 2004 Dec 2;84(23):1973-6.
To investigate the HIV-1 nef specific CD8(+) T cell responses in long-term nonprogressor (LTNP) and AIDS patient in China.
Peripheral blood mononuclear cells were collected from 7 LTNPs and 9 AIDS patients, 12 males and 4 females, aged 37 (27 approximately 55). A panel of 26 overlapping peptides spanning HIV-1 nef constituting 3 peptide pools was used as antigens. The HIV-1 nef specific CD8(+) T cell responses of the LTNPs and AIDS patients were examined by IFN- ELISPOT assay. The amount of CD4(+) T cell and viral load were detected. The relationships between HIV-1 nef specific CD8(+) T cell responses and CD4(+) T cell counts and viral loads were studied.
The strength of HIV-1 nef specific CD8(+) T cell responses of LTNP group was 404 +/- 33 SFC/10(6) PBMCs, significantly higher than that of the AIDS group (59 +/- 121 SFC/10(6) PBMCs). The strength of HIV-1 nef specific CD8(+) T cell responses was positively correlated with the CD4(+) T cell count. No relationship between the strength of CD8(+) T cell responses and viral load was found.
HIV-1 nef specific CD8(+) T cell responses may play an important role in preventing disease progression of HIV infection. There is a cross immune response between the major HIV-1 strains in Europe and those in China.
研究中国长期不进展者（LTNP）和艾滋病患者中HIV-1 nef特异性CD8(+) T细胞反应。
收集7例LTNP和9例艾滋病患者的外周血单个核细胞，共12例男性和4例女性，年龄37岁（27至55岁）。一组由26个重叠肽组成的涵盖HIV-1 nef的3个肽池用作抗原。通过IFN-ELISPOT试验检测LTNP和艾滋病患者的HIV-1 nef特异性CD8(+) T细胞反应。检测CD4(+) T细胞数量和病毒载量。研究HIV-1 nef特异性CD8(+) T细胞反应与CD4(+) T细胞计数及病毒载量之间的关系。
LTNP组HIV-1 nef特异性CD8(+) T细胞反应强度为404±33 SFC/10(6) PBMCs，显著高于艾滋病组（59±121 SFC/10(6) PBMCs）。HIV-1 nef特异性CD8(+) T细胞反应强度与CD4(+) T细胞计数呈正相关。未发现CD8(+) T细胞反应强度与病毒载量之间的关系。
HIV-1 nef特异性CD8(+) T细胞反应可能在预防HIV感染疾病进展中起重要作用。欧洲主要HIV-1毒株与中国的毒株之间存在交叉免疫反应。
