Busscher H J, Doornbusch G I, Van der Mei H C
Laboratory for Materia Technica, University of Groningen, The Netherlands.
J Dent Res. 1992 Mar;71(3):491-500. doi: 10.1177/00220345920710031301.
Deposition and adhesion to glass with and without a salivary coating in a parallel-plate flow chamber were studied with four strains of mutans streptococci. Stationary-state adhesion of the strains to uncoated glass ranged from 0.3 x 10(6) cm-2 (Streptococcus rattus HG218) to 12.7 x 10(6) cm-2 (Streptococcus sobrinus HG1025) and generally decreased after saliva coating of the glass. The poor adhesion found for S. rattus HG218 to both uncoated and saliva-coated glass could be due to its relatively high negative surface-charge. Deposition efficiencies of all strains were greater than or equal to 1 for uncoated glass and decreased greatly after saliva coating of the glass. Possibly, adhesion to a saliva coating is less efficient and more time-consuming than that to uncoated glass, because stereochemical groups in the pellicle and on the cell surfaces may have to re-arrange before an effective interaction can occur. Desorption rates, measured 1000 s and 5000 s after the start of an experiment, decreased by a factor of ten upon a five-fold increase in contact time, indicative of a two-phase adhesion process. Of the four strains studied, only Streptococcus cricetus HG737 showed a minor positive cooperativity on saliva-coated glass, possibly mediated by surface appendages observed by transmission electron microscopy on negatively-stained cells. Retention of adhering bacteria was strain-dependent on uncoated glass, but was identical for all strains on saliva-coated glass, which suggests that the structure and composition of the pellicle may be more important with respect to the retention of adhering cells than the cell-surface properties themselves.
利用四株变形链球菌菌株，研究了在平行板流动腔中，有唾液涂层和无唾液涂层的情况下，细菌在玻璃上的沉积和黏附情况。这些菌株在未涂层玻璃上的稳态黏附范围为0.3×10⁶ cm⁻²（鼠链球菌HG218）至12.7×10⁶ cm⁻²（远缘链球菌HG1025），在玻璃涂上唾液后，黏附通常会下降。鼠链球菌HG218在未涂层和涂有唾液的玻璃上黏附性差，可能是由于其相对较高的负表面电荷。对于未涂层玻璃，所有菌株的沉积效率均大于或等于1，在玻璃涂上唾液后，沉积效率大幅下降。可能是因为与未涂层玻璃相比，与唾液涂层的黏附效率较低且更耗时，因为在有效相互作用发生之前，薄膜和细胞表面的立体化学基团可能需要重新排列。在实验开始1000秒和5000秒后测量的解吸率，在接触时间增加五倍时下降了一个数量级，表明存在两相黏附过程。在所研究的四株菌株中，只有仓鼠链球菌HG737在涂有唾液的玻璃上表现出轻微的正协同作用，这可能是由透射电子显微镜在负染色细胞上观察到的表面附属物介导的。在未涂层玻璃上，黏附细菌的保留情况因菌株而异，但在涂有唾液的玻璃上，所有菌株的情况相同，这表明就黏附细胞的保留而言，薄膜的结构和组成可能比细胞表面特性本身更重要。
