编码磷酸结合蛋白PstS1和PstS2的基因发生破坏的结核分枝杆菌在磷酸盐摄取方面存在缺陷，并表现出体内毒力降低。
Mycobacterium tuberculosis with disruption in genes encoding the phosphate binding proteins PstS1 and PstS2 is deficient in phosphate uptake and demonstrates reduced in vivo virulence.
作者信息
Peirs Priska, Lefèvre Philippe, Boarbi Samira, Wang Xiao-Ming, Denis Olivier, Braibant Martine, Pethe Kevin, Locht Camille, Huygen Kris, Content Jean
机构信息
Pasteur Institute of Brussels, Engelandstraat 642, B-1180-Brussels, Belgium.
出版信息
Infect Immun. 2005 Mar;73(3):1898-902. doi: 10.1128/IAI.73.3.1898-1902.2005.
Abstract
By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding proteins. In a mouse infection model, both mutants were attenuated in virulence, suggesting that M. tuberculosis encounters limiting phosphate concentrations during its intracellular life span.
摘要
通过测量基因敲除pstS1和pstS2基因的结核分枝杆菌菌株对磷酸盐的摄取，我们发现这些pstS基因编码高亲和力磷酸盐结合蛋白。在小鼠感染模型中，两种突变体的毒力均减弱，这表明结核分枝杆菌在其细胞内存活期间会遇到磷酸盐浓度受限的情况。
相似文献
1
Mycobacterium tuberculosis with disruption in genes encoding the phosphate binding proteins PstS1 and PstS2 is deficient in phosphate uptake and demonstrates reduced in vivo virulence.
Infect Immun. 2005 Mar;73(3):1898-902. doi: 10.1128/IAI.73.3.1898-1902.2005.
2
Mutants of Mycobacterium tuberculosis lacking three of the five rpf-like genes are defective for growth in vivo and for resuscitation in vitro.
Infect Immun. 2005 May;73(5):3038-43. doi: 10.1128/IAI.73.5.3038-3043.2005.
3
senX3-independent contribution of regX3 to Mycobacterium tuberculosis virulence.
BMC Microbiol. 2014 Oct 25;14:265. doi: 10.1186/s12866-014-0265-8.
4
Inactivation of Rv2525c, a substrate of the twin arginine translocation (Tat) system of Mycobacterium tuberculosis, increases beta-lactam susceptibility and virulence.
J Bacteriol. 2006 Sep;188(18):6669-79. doi: 10.1128/JB.00631-06.
5
Mycobacterium tuberculosis ECF sigma factor sigC is required for lethality in mice and for the conditional expression of a defined gene set.
Mol Microbiol. 2004 Apr;52(1):25-38. doi: 10.1111/j.1365-2958.2003.03958.x.
6
The Mycobacterium tuberculosis protein serine/threonine kinase PknG is linked to cellular glutamate/glutamine levels and is important for growth in vivo.
Mol Microbiol. 2004 Jun;52(6):1691-702. doi: 10.1111/j.1365-2958.2004.04085.x.
7
Mycobacterium tuberculosis Phosphate Uptake System Component PstA2 Is Not Required for Gene Regulation or Virulence.
PLoS One. 2016 Aug 24;11(8):e0161467. doi: 10.1371/journal.pone.0161467. eCollection 2016.
8
The AraC family transcriptional regulator Rv1931c plays a role in the virulence of Mycobacterium tuberculosis.
Infect Immun. 2004 Sep;72(9):5483-6. doi: 10.1128/IAI.72.9.5483-5486.2004.
9
The case for hypervirulence through gene deletion in Mycobacterium tuberculosis.
Trends Microbiol. 2008 Sep;16(9):436-41. doi: 10.1016/j.tim.2008.06.003. Epub 2008 Aug 11.
10
Disruption of the ESX-5 system of Mycobacterium tuberculosis causes loss of PPE protein secretion, reduction of cell wall integrity and strong attenuation.
Mol Microbiol. 2012 Mar;83(6):1195-209. doi: 10.1111/j.1365-2958.2012.08001.x. Epub 2012 Feb 20.
引用本文的文献
1
Structural and molecular basis for phosphate recognition by SAR11 bacteria.
mBio. 2025 Aug 13:e0165425. doi: 10.1128/mbio.01654-25.
2
An exacerbated phosphate starvation response triggers glycerol utilization at acidic pH.
mBio. 2025 Jan 8;16(1):e0282524. doi: 10.1128/mbio.02825-24. Epub 2024 Nov 29.
3
Predominantly Orphan Secretome in the Lung Pathogen Revealed by a Multipronged Growth-Phase-Driven Strategy.
Microorganisms. 2024 Feb 12;12(2):378. doi: 10.3390/microorganisms12020378.
4
Antibodies as clinical tools for tuberculosis.
Front Immunol. 2023 Dec 14;14:1278947. doi: 10.3389/fimmu.2023.1278947. eCollection 2023.
5
Rv0928 protein facilitates macrophage control of mycobacterium infection by promoting mitochondrial intrinsic apoptosis and ROS-mediated inflammation.
Front Microbiol. 2023 Oct 31;14:1291358. doi: 10.3389/fmicb.2023.1291358. eCollection 2023.
6
A novel multi-component protein vaccine ECP001 containing a protein polypeptide antigen nPstS1 riching in T-cell epitopes showed good immunogenicity and protection in mice.
Front Immunol. 2023 Apr 21;14:1138818. doi: 10.3389/fimmu.2023.1138818. eCollection 2023.
7
Proteome profiling reveals changes in energy metabolism, transport and antioxidation during drought stress in Nostoc flagelliforme.
BMC Plant Biol. 2022 Apr 1;22(1):162. doi: 10.1186/s12870-022-03542-8.
8
Quantitative proteomics reveals that dormancy-related proteins mediate the attenuation in mycobacterium strains.
Virulence. 2021 Dec;12(1):2228-2246. doi: 10.1080/21505594.2021.1965703.
9
Comparative Whole-Genome Sequence Analyses of Fusarium Wilt Pathogen ( R1, STR4 and TR4) Infecting Cavendish (AAA) Bananas in India, with a Special Emphasis on Pathogenicity Mechanisms.
J Fungi (Basel). 2021 Aug 31;7(9):717. doi: 10.3390/jof7090717.
10
Antigenic sites in SARS-CoV-2 spike RBD show molecular similarity with pathogenic antigenic determinants and harbors peptides for vaccine development.
Immunobiology. 2021 Sep;226(5):152091. doi: 10.1016/j.imbio.2021.152091. Epub 2021 Jul 13.
本文引用的文献
1
Different susceptibility of two animal species infected with isogenic mutants of Mycobacterium bovis identifies phoT as having roles in tuberculosis virulence and phosphate transport.
Microbiology (Reading). 2003 Nov;149(Pt 11):3203-3212. doi: 10.1099/mic.0.26469-0.
2
Life on the inside for Mycobacterium tuberculosis.
Nat Med. 2003 Nov;9(11):1356-7. doi: 10.1038/nm1103-1356.
3
Crystal structure of M tuberculosis ABC phosphate transport receptor: specificity and charge compensation dominated by ion-dipole interactions.
Structure. 2003 Jul;11(7):765-74. doi: 10.1016/s0969-2126(03)00109-6.
4
The complete genome sequence of Mycobacterium bovis.
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7877-82. doi: 10.1073/pnas.1130426100. Epub 2003 Jun 3.
5
Characterization of PitA and PitB from Escherichia coli.
J Bacteriol. 2001 Sep;183(17):5008-14. doi: 10.1128/JB.183.17.5008-5014.2001.
6
The cell surface associated phosphatase activity of Mycobacterium bovis BCG is not regulated by environmental inorganic phosphate.
FEMS Microbiol Lett. 2001 Feb 20;195(2):121-6. doi: 10.1111/j.1574-6968.2001.tb10508.x.
7
The ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis.
FEMS Microbiol Rev. 2000 Oct;24(4):449-67. doi: 10.1111/j.1574-6976.2000.tb00550.x.
8
The Mycobacterium bovis homologous protein of the Mycobacterium tuberculosis serine/threonine protein kinase Mbk (PknD) is truncated.
FEMS Microbiol Lett. 2000 Jul 15;188(2):135-9. doi: 10.1111/j.1574-6968.2000.tb09184.x.
9
Unravelling the mysteries of virulence gene regulation in Salmonella typhimurium.
Mol Microbiol. 2000 Jun;36(5):1024-33. doi: 10.1046/j.1365-2958.2000.01961.x.
10
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol. 1999 Apr 2;287(3):467-84. doi: 10.1006/jmbi.1999.2624.
Zotero 插件