Rivera Norma, Ibarra Froylán, Zepeda Armando, Fortoul Teresa, Cantó Germinal, Hernández Alicia, Castillo Rafael
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de Mexico, 04510, Mexico City, Mexico.
Parasitol Res. 2005 Apr;95(6):379-82. doi: 10.1007/s00436-005-1304-z. Epub 2005 Feb 25.
The damage to the tegument of 3-week-old Fasciola hepatica was evaluated by scanning electron microscopy (SEM) following treatment with the 5-chloro-2-methylthio-6-(1-naphtyloxy)-1H-benzimidazole (called compound alpha) in its natural host. For the present study, flukes were raised in pelibuey sheep infected orally with metacercariae of F. hepatica; the parasites were recovered from the liver of the sacrificed sheep after 6, 12 and 24 h of treatment with compound alpha. At 6 h of treatment, the flukes showed some lesions on the ventral surface of the anterior region, such as a swollen tegument and blebs. At 12 h after treatment, the specimens showed structural disorganization and spine loss in the ventral anterior region. The tegument of the flukes treated for 24 h was completely lost in some areas of the ventral surface, leaving an exposed basal lamina. The tegument of immature F. hepatica might be a target organ for compound alpha to exert its fasciolicide effect.
在用5-氯-2-甲硫基-6-(1-萘氧基)-1H-苯并咪唑（称为化合物α）处理其天然宿主后，通过扫描电子显微镜（SEM）评估3周龄肝片吸虫体表的损伤情况。在本研究中，将肝片吸虫的囊蚴经口感染佩利布埃羊来饲养吸虫；在用化合物α处理6、12和24小时后，从处死的羊的肝脏中回收寄生虫。在处理6小时时，吸虫在前部区域的腹面出现一些损伤，如体表肿胀和水泡。处理12小时后，标本在腹面前部区域出现结构紊乱和棘刺脱落。处理24小时的吸虫体表在腹面的某些区域完全消失，留下暴露的基膜。未成熟肝片吸虫的体表可能是化合物α发挥杀吸虫作用的靶器官。
