Ibarra F, Montenegro N, Vera Y, Boulard C, Quiroz H, Flores J, Ochoa P
Proyecto Fasciolosis, CENID-Parasitología/INIFAP/SAGAR, Carretera Cuernavaca-Cuautla, Morelos, Mexico.
Vet Parasitol. 1998 Jun 30;77(4):229-36. doi: 10.1016/s0304-4017(98)00111-3.
The aim of the present study was to compare the sensitivity, specificity and usefulness of the DIG-ELISA, DOT-ELISA and Indirect ELISA tests for determining the seroprevalence of fasciolosis in cattle under tropical conditions in Mexico. To standardize the tests, positive and negative sera to F. hepatica from 88 Holstein Freisian adult cows located in an enzootic area of fascioliosis and 88 crossbred adult cattle from a fluke-free area were used. For the epidemiological study, 85 crossbred cattle between 1 to 7 years of age were used. Animals were bled every two months, from March 1995 to September 1996 and the sera obtained were stored at -70 degrees C, until used. Indirect ELISA showed a sensitivity of 96.5% and a specificity of 98.8%, DIG-ELISA 97.5% and 80.0% and DOT-ELISA 93.1% and 95.4%, respectively. During 1995, Indirect ELISA yielded the highest levels of IgG anti-F. hepatica antibodies. However, in 1996, after animal treatment with triclabendazole, DIG-ELISA tended to show higher percentages of antibody-positive animals, but it was not significantly different (p>0.05) from the other tests. Comparisons made in parallel to the faecal sedimentation test demonstrated that all serological tests detected higher percentages of positive animals. Only one serum out of ten (10%) of Paramphistomum spp. cross-reacted with the DOT-ELISA test, but no cross-reaction was observed with sera from animals with other parasites. All ELISA tests were highly sensitive and specific; they may be recommended for use in seroepidemiological surveys for F. hepatica.
本研究的目的是比较双抗体夹心酶联免疫吸附测定（DIG-ELISA）、斑点酶联免疫吸附测定（DOT-ELISA）和间接酶联免疫吸附测定在墨西哥热带条件下测定牛肝片吸虫病血清流行率时的敏感性、特异性和实用性。为使试验标准化，使用了来自肝片吸虫病疫区的88头荷斯坦弗里生成年奶牛以及来自无吸虫地区的88头杂交成年牛的肝片吸虫阳性和阴性血清。在流行病学研究中，使用了85头1至7岁的杂交牛。从1995年3月至1996年9月，每两个月采集一次动物血液，所获血清保存在-70℃，直至使用。间接酶联免疫吸附测定的敏感性为96.5%，特异性为98.8%；DIG-ELISA的敏感性为97.5%，特异性为80.0%；DOT-ELISA的敏感性为93.1%，特异性为95.4%。1995年期间，间接酶联免疫吸附测定产生的抗肝片吸虫IgG抗体水平最高。然而，1996年在用三氯苯达唑治疗动物后，DIG-ELISA显示抗体阳性动物的百分比更高，但与其他试验相比无显著差异（p>0.05）。与粪便沉淀试验平行进行的比较表明，所有血清学试验检测到的阳性动物百分比更高。在十种双口吸虫属血清中，只有一种（10%）与DOT-ELISA试验发生交叉反应，但未观察到与其他寄生虫感染动物的血清发生交叉反应。所有酶联免疫吸附测定试验均具有高度敏感性和特异性；可推荐用于肝片吸虫的血清流行病学调查。
