Ko Samuel, Chu Kent-Man, Luk John Moon-Ching, Wong Bonnie Wing-Yan, Yuen Siu-Tsan, Leung Suet-Yi, Wong John
Department of Surgery, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.
J Pathol. 2005 Apr;205(5):615-22. doi: 10.1002/path.1741.
CDX2 and liver-intestine (LI)-cadherin are intestine-specific markers and both are physiologically expressed in the small intestine and colon. Recent studies have demonstrated that CDX2 regulates LI-cadherin gene (CDH17) expression in colorectal cancer. The present study investigated the relationship of CDX2 and LI-cadherin expression in gastric cancer. One hundred and nine pairs of tumour and non-cancerous gastric mucosa were collected from gastrectomy specimens. Protein expression levels of CDX2 and LI-cadherin were determined by immunohistochemical staining. Semi-quantitative RT-PCR showed that the mRNAs of both CDX2 and CDH17 were highly expressed in tumour compared with non-cancerous mucosa. Overexpression of CDX2 was significantly associated with CDH17 in gastric adenocarcinoma. Furthermore, the expression of CDX2 and LI-cadherin proteins was strongly coupled in intestinal metaplasia. In conclusion, overexpression of CDH17 is significantly associated with CDX2.
CDX2和肝肠（LI）-钙黏蛋白是肠道特异性标志物，二者均在小肠和结肠中生理性表达。近期研究表明，CDX2在结直肠癌中调控LI-钙黏蛋白基因（CDH17）的表达。本研究调查了CDX2与LI-钙黏蛋白在胃癌中的表达关系。从胃切除标本中收集了109对肿瘤及癌旁胃黏膜。通过免疫组织化学染色测定CDX2和LI-钙黏蛋白的蛋白表达水平。半定量逆转录聚合酶链反应显示，与癌旁黏膜相比，CDX2和CDH17的信使核糖核酸在肿瘤中均高表达。在胃腺癌中，CDX2的过表达与CDH17显著相关。此外，在肠化生中，CDX2和LI-钙黏蛋白的蛋白表达紧密相关。总之，CDH17的过表达与CDX2显著相关。
