在聚二甲基硅氧烷微流控芯片中进行等电聚焦。
Isoelectric focusing in a poly(dimethylsiloxane) microfluidic chip.
作者信息
Cui Huanchun, Horiuchi Keisuke, Dutta Prashanta, Ivory Cornelius F
机构信息
Department of Chemical Engineering, and School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USA.
出版信息
Anal Chem. 2005 Mar 1;77(5):1303-9. doi: 10.1021/ac048915+.
This paper reports the application of ampholyte-based isoelectric focusing in poly(dimethylsiloxane) (PDMS) using methylcellulose (MC) to reduce electroosmosis and peak drift. Although the characteristics of PDMS make it possible to fabricate microfluidic chips using soft lithography, unstable electroosmotic flow (EOF) and cathodic drift are significant problems when this medium is used. This paper demonstrates that EOF is greatly reduced in PDMS by applying a dynamic coat of MC to the channel walls and that higher concentrations of MC can be used to increase the viscosity of the electrode solutions in order to suppress pH gradient drift and reduce "compression"of the pH gradient. To illustrate the effect of MC on performance, several fluorescent proteins were focused in microchip channels 5 microm deep by 300 microm wide by 2 cm long in 3-10 min using broad-range ampholytes at electric field strengths ranging from 25 to 100 V/cm.
本文报道了基于两性电解质的等电聚焦在聚二甲基硅氧烷（PDMS）中的应用，其中使用甲基纤维素（MC）来减少电渗和峰漂移。尽管PDMS的特性使得使用软光刻技术制造微流控芯片成为可能，但当使用这种介质时，不稳定的电渗流（EOF）和阴极漂移是严重的问题。本文表明，通过在通道壁上施加动态的MC涂层，PDMS中的EOF可大大降低，并且可以使用更高浓度的MC来增加电极溶液的粘度，以抑制pH梯度漂移并减少pH梯度的“压缩”。为了说明MC对性能的影响，使用宽范围两性电解质，在电场强度为25至100 V/cm的条件下，在深度为5微米、宽度为300微米、长度为2厘米的微芯片通道中，在3至10分钟内对几种荧光蛋白进行了聚焦。
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