Pan Jia-hua, Lou Wan-ling, Zhou Hao-quan, Shen Huai-yun, Chen Lan-ju
Department of Pediatrics, Anhui Provincial Hospital, Hefei, 230001 China.
Zhonghua Er Ke Za Zhi. 2004 Dec;42(12):932-5.
The protection rate of inoculation with BCG vaccine is only 50 percent, and most of patients with tuberculosis had a history of BCG vaccine inoculation. Adenosine (ADO) has an immunomodulating effect; it promotes immune reaction by increasing number of macrophage and enhancing phagocytosis. The present study was designed to investigate if combined use of adenosine with BCG enhances the anti-Mycobacterium tuberculosis effect of macrophage in mice.
Fifty BALB/C mice were divided randomly into 3 groups: BCG group (n = 21), BCG plus ADO group (n = 21) and control group (n = 8). The mice in BCG and BCG plus ADO groups were inoculated with 0.1 ml BCG intradermally and the mice in BCG plus ADO group were injected intraperitoneally with ADO 30 mg/(kg.d) for 5 days. The mice in BCG group and control group were injected with NS 0.1 ml/d for 5 days. Six weeks after the last injection, all mice were challenged with intravenous 1 x 10(6) CFU human Mycobacterium tuberculosis virulent strain. After challenging, lung and spleen specimens were taken at the 10th, 20th and 30th days from the mice of BCG and BCG plus ADO groups and at the 30th day from mice in control group. The pathological examinations of lung and spleen sections were performed after HE staining and acid-fast staining, and detection of cell apoptosis was also performed.
Consolidation with neutrophil infiltration was found in most of the lung tissue taken at the day 30; there were a lot of tuberculous granulomas and Mycobacterium tuberculosis in the lungs of control group. The alveolar septum in BCG gradually became wide and in interstitium lymphocyte infiltration dominated, and there were less tuberculous granulomas but there were large number of Mycobacterium tuberculosis in the lungs from 10th to 30th days after challenging. The widening of alveolar septum and consolidation of lung tissue in BCG plus ADO group became milder with monocytes infiltration, and there were few tuberculosis granulomas and Mycobacterium tuberculosis in the lungs from 10th to 30th days after challenging.
ADO could increase the number of monocyte-macrophages and promoted anti-bacterial effects of these cells.
卡介苗接种的保护率仅为50%，且大多数结核病患者有卡介苗接种史。腺苷（ADO）具有免疫调节作用；它通过增加巨噬细胞数量和增强吞噬作用来促进免疫反应。本研究旨在探讨腺苷与卡介苗联合使用是否能增强小鼠巨噬细胞对结核分枝杆菌的抗结核作用。
将50只BALB/C小鼠随机分为3组：卡介苗组（n = 21）、卡介苗加ADO组（n = 21）和对照组（n = 8）。卡介苗组和卡介苗加ADO组小鼠皮内接种0.1 ml卡介苗，卡介苗加ADO组小鼠腹腔注射ADO 30 mg/(kg·d)，共5天。卡介苗组和对照组小鼠每天注射0.1 ml生理盐水，共5天。最后一次注射后6周，所有小鼠静脉注射1×10(6) CFU人结核分枝杆菌强毒株进行攻击。攻击后，在第10、20和30天从卡介苗组和卡介苗加ADO组小鼠中采集肺和脾标本，在第30天从对照组小鼠中采集肺和脾标本。对肺和脾切片进行HE染色和抗酸染色后进行病理检查，并检测细胞凋亡。
在第30天采集的大多数肺组织中发现有中性粒细胞浸润的实变；对照组肺中有大量结核结节和结核分枝杆菌。卡介苗组肺泡间隔逐渐增宽，间质以淋巴细胞浸润为主，攻击后10至30天肺中结核结节较少，但有大量结核分枝杆菌。卡介苗加ADO组肺泡间隔增宽和肺组织实变较轻，有单核细胞浸润，攻击后10至30天肺中结核结节和结核分枝杆菌较少。
ADO可增加单核细胞-巨噬细胞数量并促进这些细胞的抗菌作用。
