Song Yi, Wu Guan, Xin Dian-qi, Na Yan-qun
Institute of Urology, the Department of Urology, First Hospital of Peking University, Beijing 100034, China.
Zhonghua Wai Ke Za Zhi. 2004 Dec 7;42(23):1453-6.
To study the paracrine effect of the neuroendocrine differentiation cells and its influence on androgen receptor expression of prostate carcinoma cells.
Established an in vitro induced model of neuroendocrine differentiation of prostate carcinoma (LNCaP(NE), PC-3M(NE)), and investigate the proliferation effect of neuroendocrine phenotype cells (LNCaP(NE), PC-3M(NE)) on other non-neuroendocrine phenotype cells (LNCaP, PC-3M) by feeding non-neuroendocrine phenotype cells with the medium pre-incubated with induced neuroendocrine phenotype cells. It was also tested the regulation of androgen receptor mRNA and androgen receptor protein expression of LNCaP(NE) cells on LNCaP cells by reverse transcriptase polymerase chain reaction and Western Blot in the presence or absence of androgens.
The medium pre-incubated with PC-3M(NE) cells could promote the proliferation of PC-3M cells. The medium pre-incubated with LNCaP(NE) cells could promote the proliferation of LNCaP cells, and reduce the expression of androgen receptor in the latter in the absence of androgens, but the negative results were observed in the presence of androgens.
The neuroendocrine phenotype cells of prostate cancer can reduce the expression of androgen receptor in prostate cancer cells and promote them to proliferate by means of paracrine in the blockade of androgens.
研究神经内分泌分化细胞的旁分泌作用及其对前列腺癌细胞雄激素受体表达的影响。
建立前列腺癌神经内分泌分化的体外诱导模型（LNCaP(NE)、PC-3M(NE)），用诱导的神经内分泌表型细胞预孵育的培养基培养非神经内分泌表型细胞（LNCaP、PC-3M），观察神经内分泌表型细胞（LNCaP(NE)、PC-3M(NE)）对其他非神经内分泌表型细胞的增殖作用。同时在有无雄激素存在的情况下，通过逆转录聚合酶链反应和蛋白质免疫印迹法检测LNCaP(NE)细胞对LNCaP细胞雄激素受体mRNA和雄激素受体蛋白表达的调控。
PC-3M(NE)细胞预孵育的培养基能促进PC-3M细胞增殖。LNCaP(NE)细胞预孵育的培养基能促进LNCaP细胞增殖，且在无雄激素时可降低后者雄激素受体的表达，但在有雄激素时结果为阴性。
前列腺癌神经内分泌表型细胞可通过旁分泌方式在雄激素阻断的情况下降低前列腺癌细胞雄激素受体的表达并促进其增殖。
