通过γ射线辐照制备用于包埋脲酶的聚乙烯醇-聚丙烯酰胺复合聚合物膜。
Preparation of polyvinyl alcohol-polyacrylamide composite polymer membrane by gamma-irradiation for entrapment of urease.
作者信息
Jha Sandeep Kumar, D'Souza Stanislaus F
机构信息
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
出版信息
J Biochem Biophys Methods. 2005 Mar 31;62(3):215-8. doi: 10.1016/j.jbbm.2004.11.006. Epub 2004 Dec 30.
PMID:15733581
Abstract
Composite polymer membrane of polyvinyl alcohol (PVA) and acrylamide was prepared on cheesecloth support by gamma-irradiation induced free radical polymerization. The enzyme urease was entrapped in the membrane during polymerization and was cross-linked within the matrix using glutaraldehyde. The membranes could be reused a number of times without significant loss of urease activity.
摘要
通过γ射线辐照引发自由基聚合反应，在粗棉布载体上制备了聚乙烯醇（PVA）和丙烯酰胺的复合聚合物膜。在聚合过程中，脲酶被包埋在膜中，并使用戊二醛在基质内交联。这些膜可以重复使用多次，而脲酶活性不会有显著损失。
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