Yamada Kazuya, Ogata-Kawata Hiroko, Matsuura Kaoru, Miyamoto Kaoru
Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan.
FEBS Lett. 2005 Feb 28;579(6):1509-14. doi: 10.1016/j.febslet.2005.01.060.
The influence of the enhancer of split- and hairy-related protein-2 (SHARP-2) transcriptional repressor on the expression of rat phosphoenolpyruvate carboxykinase (PEPCK) gene was examined. When H4IIE cells were treated with epigallocatechin gallate, a green tea constituent, an increase in SHARP-2 mRNA levels and a decrease in PEPCK mRNA levels were observed. The adenovirus-mediated overexpression of SHARP-2 in H4IIE cells and primary cultured rat hepatocytes led to a decrease in the levels of PEPCK mRNA. Finally, when a SHARP-2 expression plasmid was transiently transfected with various reporter plasmids into MH1C1 cells, the promoter activity of a PEPCK reporter plasmid was specifically decreased. Based on these findings, we conclude that SHARP-2 is a potential repressor of PEPCK gene expression.
研究了分裂相关和毛相关蛋白2（SHARP-2）转录抑制因子对大鼠磷酸烯醇丙酮酸羧激酶（PEPCK）基因表达的影响。当用绿茶成分表没食子儿茶素没食子酸酯处理H4IIE细胞时，观察到SHARP-2 mRNA水平升高，PEPCK mRNA水平降低。腺病毒介导的SHARP-2在H4IIE细胞和原代培养的大鼠肝细胞中过表达导致PEPCK mRNA水平降低。最后，当将SHARP-2表达质粒与各种报告质粒瞬时转染到MH1C1细胞中时，PEPCK报告质粒的启动子活性特异性降低。基于这些发现，我们得出结论，SHARP-2是PEPCK基因表达的潜在抑制因子。
