Hayama Fábia H, Motta Ana C F, Silva Antonio de Padua G, Migliari Dante A
Departamento de Diagnóstico Oral, Escuela de Odontología, Universidad Federal de Paraná, Curitiba, PR, Brazil.
Med Oral Patol Oral Cir Bucal. 2005 Mar-Apr;10(2):115-22.
To compare specimen adequacy and diagnostic agreement between liquid-based preparations and conventional smears in oral lesions, and to test the viability of immunocytochemical assay in liquid-based preparations from oral carcinoma lesions.
Samples were collected from 44 patients. Conventional smears were prepared first, using a cytobrush device. Then the brush, containing the residual material, was immersed in a preservative fluid. The sample in the preservative fluid was processed according to the manufacturer directions (AutoCyte, Inc. Elon College, North Carolina, USA). Slides of both techniques were stained by Papanicolaou method. For immunocytochemical assay, a cytokeratin pool AE1/AE3 (Dako, CA, USA) was applied in liquid-based preparations from oral carcinoma lesions following the Streptavidin-biotin-peroxidase method. Fisher's exact test was used; significance was set for P < or = 0.05.
Both techniques agreed on cytologic diagnosis in every case they yielded an adequate specimen; in 3 cases conventional smear resulted in hypocellularity and therefore inadequate for analysis. On specimen analysis, the liquid-based cytology demonstrated a statistically significant, 41% overall improvement in smear thickness and 66% in cell distribution (P < or = 0.05), and a reduction in cell overlapping and presence of blood (P < or = 0.05). The cell morphology was better visualized in the liquid-based preparations. The immunocytochemical assay reactions were positive in all malignant cases, the visualization of the immunostained cells being especially clear.
Both, the liquid-based preparation and conventional smear, are diagnostically reliable; the liquid-based method showed an overall improvement on sample preservation, specimen adequacy, visualization of cell morphology and reproducibility.
比较液基制片与传统涂片在口腔病变中的标本充足率和诊断一致性，并检测免疫细胞化学分析在口腔癌病变液基制片中的可行性。
收集44例患者的样本。首先使用细胞刷装置制备传统涂片。然后将含有残留物质的刷子浸入保存液中。按照制造商说明（美国北卡罗来纳州伊隆学院的AutoCyte公司）处理保存液中的样本。两种技术的玻片均采用巴氏染色法染色。对于免疫细胞化学分析，按照链霉亲和素-生物素-过氧化物酶方法，将细胞角蛋白组合AE1/AE3（美国加利福尼亚州的达科公司）应用于口腔癌病变的液基制片中。采用Fisher精确检验；设定P≤0.05为有统计学意义。
两种技术在每例获得充足标本的病例中细胞学诊断结果一致；3例传统涂片细胞过少，因此无法进行分析。在标本分析中，液基细胞学显示涂片厚度总体有统计学意义地提高了41%，细胞分布提高了66%（P≤0.05），细胞重叠和血液存在情况减少（P≤0.05）。液基制片中细胞形态更易于观察。所有恶性病例的免疫细胞化学分析反应均为阳性，免疫染色细胞的观察尤其清晰。
液基制片和传统涂片在诊断上均可靠；液基方法在样本保存、标本充足率、细胞形态观察和可重复性方面总体有改善。
