Kim Soon-Ja, Bok Song-Hae, Lee Sangku, Lee Mi-Kyung, Park Yong Bok, Kim Hye-Jin, Choi Myung-Sook
Department of Food Science and Nutrition, Kyungpook National University, 702-701, Daegu, South Korea.
J Biochem Mol Toxicol. 2005;19(1):25-31. doi: 10.1002/jbt.20054.
A preliminary study revealed that 3,4-di(OH)-hydrocinnamate (HC), a polyphenolic compound, lowered the plasma lipids in high-cholesterol fed rats. Accordingly, this study was designed to test the lipid-lowering efficacy of a synthetic derivative, 3,4-di(OH)-phenylpropionic (L-leucine) amide (PPLA), in rats fed a high-cholesterol (1%, wt/wt) diet. As such, HC or PPLA was given as supplement to a high-cholesterol diet for 6 weeks at a dose of 0.137 mmol/100 g diet. The supplementation of HC and PPLA significantly lowered the plasma and hepatic cholesterol and triglyceride levels compared to the control group. The activities of hepatic HMG-CoA reductase (164 +/- 9.12 and 124.74 +/- 17.09 pmol/min/mg protein vs. 245.41 +/- 13.01 pmol/min/mg protein, p < 0.05) and ACAT (411.49 +/- 11.48 and 334.35 +/- 17.68 pmol/min/mg protein vs. 490.41 +/- 16.69 pmol/min/mg protein, p < 0.05) were significantly lower in the HC- and PPLA-supplemented groups than in the control group. However, PPLA was more effective in inhibiting the enzyme activities than HC. The excretion of neutral sterol was significantly higher in HC- and PPLA-supplemented groups than in the control group. Therefore, these results indicate that PPLA, a leucine-attached version of HC, exhibited a similar significant hypocholesterolemic effect to HC in rats fed a high-cholesterol diet.
一项初步研究表明，多酚类化合物3,4 - 二（羟基）肉桂酸酯（HC）可降低高胆固醇喂养大鼠的血脂。因此，本研究旨在测试合成衍生物3,4 - 二（羟基）苯丙酸（L - 亮氨酸）酰胺（PPLA）对高胆固醇（1%，重量/重量）饮食喂养大鼠的降脂效果。为此，将HC或PPLA以0.137 mmol/100 g饮食的剂量作为高胆固醇饮食的补充剂给予6周。与对照组相比，补充HC和PPLA显著降低了血浆和肝脏中的胆固醇及甘油三酯水平。补充HC和PPLA组的肝脏HMG - CoA还原酶活性（分别为164±9.12和124.74±17.09 pmol/分钟/毫克蛋白质，而对照组为245.41±13.01 pmol/分钟/毫克蛋白质，p < 0.05）和ACAT活性（分别为411.49±11.48和334.35±17.68 pmol/分钟/毫克蛋白质，而对照组为490.41±16.69 pmol/分钟/毫克蛋白质，p < 0.05）明显低于对照组。然而，PPLA在抑制酶活性方面比HC更有效。补充HC和PPLA组的中性固醇排泄量明显高于对照组。因此，这些结果表明，PPLA作为HC的亮氨酸连接形式，在高胆固醇饮食喂养的大鼠中表现出与HC相似的显著降胆固醇作用。
