Dabrowska Krystyna, Opolski Adam, Wietrzyk Joanna, Switala-Jelen Kinga, Godlewska Joanna, Boratynski Janusz, Syper Danuta, Weber-Dabrowska Beata, Gorski Andrzej
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114 Wroclaw, Poland.
Anticancer Res. 2004 Nov-Dec;24(6):3991-5.
Previously, we have shown the ability of the bacteriophage T4 and its substrain HAP1 (selected for a higher affinity to melanoma cells) to reveal antimetastatic activity in a mouse melanoma model. Here, we investigated the potential phage anticancer activity in primary tumour models.
Mice were inoculated subcutaneously with B16 or LLC cells (collected from in vitro culture). Bacteriophages T4 and HAP1 were injected intraperitoneally daily (8 x 10(8)pfu/mouse, except the experiment concerning the dose-dependence).
Treatment with purified preparations of bacteriophage T4 resulted in significant reduction of tumour size, the effect being dose-dependent. HAP1 was more effective than T4 and its activity was also dose-dependent. Parallel experiments with non-purified bacteriophage lysates resulted in significant stimulation of tumour growth.
These data suggest that purified bacteriophages may inhibit tumour growth, a phenomenon with potentially important clinical implications in oncology.
此前，我们已证明噬菌体T4及其亚菌株HAP1（因对黑色素瘤细胞具有更高亲和力而被筛选出来）在小鼠黑色素瘤模型中具有抗转移活性。在此，我们研究了噬菌体在原发性肿瘤模型中的潜在抗癌活性。
将B16或LLC细胞（从体外培养物中收集）皮下接种到小鼠体内。每天腹腔注射噬菌体T4和HAP1（8×10⁸噬菌斑形成单位/小鼠，剂量依赖性实验除外）。
用纯化的噬菌体T4制剂治疗可显著减小肿瘤大小，且该效应具有剂量依赖性。HAP1比T4更有效，其活性也具有剂量依赖性。用未纯化的噬菌体裂解物进行的平行实验导致肿瘤生长显著受刺激。
这些数据表明纯化的噬菌体可能抑制肿瘤生长，这一现象在肿瘤学中可能具有重要的临床意义。
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