Ozbilgin M Kemal, Polat S, Mete U O, Tap O, Kaya M
Faculty of Medicine, Celal Bayar University.
J Investig Allergol Clin Immunol. 2004;14(4):320-8.
The antigen presenting cells (APCs) with special interest to dendritic cells (DC), were investigated in 28 hypertrophic and 10 control pharyngeal tonsils of children by histochemistry, immunohistochemistry and electron microscopy. In this study, we are trying to clarify the function and classification of APC in pharyngeal tonsils using morphologic criteria, Human Leukocyte Antigen Monoclonal Antibody (HLA-DR MoAb), which is specific for APCs, and acid phosphatase (APh) reacting with both phagosomes and lysosomes. The surface epithelium of the patient group examined by light microscopy, heavy infiltration of lymphocytes, degenerated columnar cells and a few HLA-DR MoAb (+) columnar cells was observed. Additionally, a significant number of APCs which were Langerhans cells (LCs), interdigitating dendritic cell (IDC), follicular dendritic cell (FDC) and macrophages were stained with both HLA-DR MoAb and APh in the epithelial, interfollicular-subepithelial and follicular areas. Ultrastructural examinations revealed that lymphocytes, macrophages, LC and M cells were found among the surface columnar epithelial cells of the patient group. The interactions between M cells and LC suggested that M cells probably passed antigens from surface to LC. In the interfollicular-subepithelial areas of the hypertrophic pharyngeal tonsil, IDCs were in close contact with lymphocytes, macrophages and plasma cells. Seven types of FDCs (FDC-1 - FDC-7) were recognised according to their ultrastructural appearances. Differentiated FDCs (FDC-4) were also in close contact with each active subtype of FDCs in follicular areas besides lymphocytes. These findings supported the idea that although the pharyngeal tonsils contained several types of active APCs, only DC were in close contact with immunocompetent cells and the other APC's. Therefore, these morphologic appearances of DC could be a sign of function to initiate the immune response of the pharyngeal tonsil.
通过组织化学、免疫组织化学和电子显微镜技术，对28例儿童肥大性咽扁桃体和10例对照咽扁桃体中特别关注的抗原呈递细胞（APC），尤其是树突状细胞（DC）进行了研究。在本研究中，我们试图利用形态学标准、对APC特异的人白细胞抗原单克隆抗体（HLA - DR MoAb）以及与吞噬体和溶酶体均反应的酸性磷酸酶（APh）来阐明咽扁桃体中APC的功能和分类。通过光学显微镜检查患者组的表面上皮，观察到淋巴细胞大量浸润、柱状细胞变性以及少数HLA - DR MoAb（+）柱状细胞。此外，在上皮、滤泡间 - 上皮下和滤泡区域，大量呈APC的朗格汉斯细胞（LC）、交错突细胞（IDC）、滤泡树突状细胞（FDC）和巨噬细胞被HLA - DR MoAb和APh双重染色。超微结构检查显示，在患者组的表面柱状上皮细胞之间发现了淋巴细胞、巨噬细胞、LC和M细胞。M细胞与LC之间的相互作用表明，M细胞可能将抗原从表面传递给LC。在肥大性咽扁桃体的滤泡间 - 上皮下区域，IDC与淋巴细胞、巨噬细胞和浆细胞紧密接触。根据超微结构外观识别出7种类型的FDC（FDC - 1 - FDC - 7）。除淋巴细胞外，分化的FDC（FDC - 4）在滤泡区域也与各活性亚型的FDC紧密接触。这些发现支持了这样一种观点，即尽管咽扁桃体含有几种类型的活性APC，但只有DC与免疫活性细胞及其他APC紧密接触。因此，DC的这些形态学表现可能是启动咽扁桃体免疫反应功能的一个标志。
