Lin Shih-Yi, Sheu Wayne Huey-Herng, Chen Wen-Yin, Lee Fa-Yauh, Huang Ching-Jang
Division of Endocrinology and Metabolism, Department of Medicine, Taichung Veterans General Hospital, Taichung, Taiwan.
Mol Cell Endocrinol. 2005 Mar 31;232(1-2):1-8. doi: 10.1016/j.mce.2005.01.006.
Resistin, an adipose-derived polypeptide hormone, is proposed as a candidate of insulin resistance, although its roles in inhibiting adipogenesis and in inflammation have also been suggested. Liver cirrhosis is characterized by elevated circulating proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-alpha), hyperinsulinemia and insulin resistance. The study aimed to examine resistin expression and its association with insulin and TNF-alpha in a cirrhotic rat model using bile duct ligation (BDL). The BDL-induced cirrhotic rats showed significantly lower fat mass, insulin sensitivity and elevated plasma insulin and TNF-alpha compared to sham animals. In addition, epididymal TNF-alpha and resistin mRNA and protein levels were higher in cirrhotic rats. In normal control rats, in vivo insulin infusion and ex vivo administration of TNF-alpha to cultured fat pads increased resistin gene expression significantly. These results implied that hyperinsulinemia and increased TNF-alpha levels might upregulate adipose resistin gene in BDL-induced liver cirrhosis. Further study is necessary to document the role of resistin in metabolic abnormalities of liver cirrhosis.
抵抗素是一种由脂肪组织分泌的多肽激素，尽管其在抑制脂肪生成和炎症方面的作用也已被提及，但它仍被认为是胰岛素抵抗的一个候选因素。肝硬化的特征是循环促炎细胞因子水平升高，包括肿瘤坏死因子-α（TNF-α）、高胰岛素血症和胰岛素抵抗。本研究旨在通过胆管结扎（BDL）建立肝硬化大鼠模型，检测抵抗素的表达及其与胰岛素和TNF-α的关系。与假手术动物相比，BDL诱导的肝硬化大鼠脂肪量显著降低，胰岛素敏感性降低，血浆胰岛素和TNF-α水平升高。此外，肝硬化大鼠附睾组织中TNF-α、抵抗素mRNA和蛋白水平均较高。在正常对照大鼠中，体内胰岛素输注以及对培养的脂肪垫进行体外TNF-α给药均显著增加了抵抗素基因的表达。这些结果表明，高胰岛素血症和TNF-α水平升高可能上调BDL诱导的肝硬化大鼠脂肪组织中抵抗素基因的表达。有必要进一步研究以证实抵抗素在肝硬化代谢异常中的作用。
