胸腺细胞发育过程中沉默染色质的组装。

Assembly of silent chromatin during thymocyte development.

作者信息

Su Ruey-Chyi, Sridharan Rupa, Smale Stephen T

机构信息

Howard Hughes Medical Institute, Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, USA.

出版信息

Semin Immunol. 2005 Apr;17(2):129-40. doi: 10.1016/j.smim.2005.01.003.

DOI:10.1016/j.smim.2005.01.003

PMID:15737574

Abstract

Epigenetic events that contribute to the assembly and maintenance of silent chromatin structures have been defined through genetic, molecular, and cytological studies in a variety of eukaryotic model organisms. However, the precise cascade of events responsible for converting a developmentally regulated gene from an active euchromatic state to a heritably silent heterochromatic state remains to be elucidated. To establish a molecular framework for studying this cascade, we examined the temporal order of events associated with silencing of the murine terminal transferase (Dntt) gene during thymocyte maturation. This article describes our findings in the context of current knowledge of gene silencing mechanisms.

摘要

通过对多种真核模式生物进行的遗传学、分子学和细胞学研究，已经明确了促成沉默染色质结构组装和维持的表观遗传事件。然而，负责将一个受发育调控的基因从活跃的常染色质状态转变为可遗传的沉默异染色质状态的精确事件级联仍有待阐明。为了建立研究这一级联反应的分子框架，我们研究了胸腺细胞成熟过程中与小鼠末端转移酶（Dntt）基因沉默相关事件的时间顺序。本文结合基因沉默机制的现有知识描述了我们的研究结果。