用于黑腹果蝇基因操作的新兴技术。

Emerging technologies for gene manipulation in Drosophila melanogaster.

作者信息

Venken Koen J T, Bellen Hugo J

机构信息

Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA.

出版信息

Nat Rev Genet. 2005 Mar;6(3):167-78. doi: 10.1038/nrg1553.

DOI:10.1038/nrg1553

PMID:15738961

Abstract

The popularity of Drosophila melanogaster as a model for understanding eukaryotic biology over the past 100 years has been accompanied by the development of numerous tools for manipulating the fruitfly genome. Here we review some recent technologies that will allow Drosophila melanogaster to be manipulated more easily than any other multicellular organism. These developments include the ability to create molecularly designed deletions, improved genetic mapping technologies, strategies for creating targeted mutations, new transgenic approaches and the means to clone and modify large fragments of DNA.

摘要

在过去的100年里，黑腹果蝇作为理解真核生物生物学的模型广受欢迎，与此同时，用于操纵果蝇基因组的众多工具也得到了发展。在此，我们回顾一些最新技术，这些技术将使黑腹果蝇比其他任何多细胞生物都更容易被操纵。这些进展包括创建分子设计缺失的能力、改进的遗传图谱技术、创建靶向突变的策略、新的转基因方法以及克隆和修饰大片段DNA的手段。