Fürstenberg Alexandre, Vauthey Eric
Department of Physical Chemistry, University of Geneva, 30 Quai Ernest-Ansermet, 1211, Geneve 4, Switzerland.
Photochem Photobiol Sci. 2005 Mar;4(3):260-7. doi: 10.1039/b418188c. Epub 2005 Feb 3.
The photophysics of the dye Lucifer Yellow ethylenediamine (LYen) has been investigated in various polar solvents. The main deactivation pathways of its first singlet excited state are the fluorescence and the intersystem crossing. In water, non-radiative decay by intermolecular proton transfer becomes a significant deactivation channel. The early fluorescence dynamics, which was investigated in liquids and in reverse micelles, was found to depend substantially on the environment. An important static quenching of LYen by tryptophan and indole occurring in the subpicosecond timescale was observed. The use of the fluorescence dynamics of LYen as a local probe is illustrated by preliminary results obtained with a biotinylated Lucifer Yellow derivative complexed with avidin.
已在各种极性溶剂中研究了染料荧光素黄乙二胺（LYen）的光物理性质。其第一单线态激发态的主要失活途径是荧光和系间窜越。在水中，分子间质子转移引起的非辐射衰变成为一个重要的失活通道。在液体和反胶束中研究的早期荧光动力学被发现很大程度上取决于环境。观察到在亚皮秒时间尺度上色氨酸和吲哚对LYen有重要的静态猝灭作用。用与抗生物素蛋白复合的生物素化荧光素黄衍生物获得的初步结果说明了将LYen的荧光动力学用作局部探针的情况。
