Zhao J, Wang C, Sun X
Henan University, Kaifeng, 475001.
Se Pu. 1997 Nov;15(6):544-5.
Solanesol, a terpene compound mainly isolated from tobacco, is an important intermediate of medicines such as vitamin K2 and coenzyme Q10. This paper presents an HPLC method for the determination of solanesol in the extracts of tobacco leaves. Solanesol is separated completely on a Zorbax-SIL column, 250 mm x 4.6 mm i.d., by using a mixture of n-hexane, isopropyl ether and ethyl acetate (6:3:1) as mobile phase and determined refractometrically. The isopropyl ether makes the chromatographic peak sharp and the addition of ethyl acetate can prevent the column from blockade during the analysis, and the appropriate temperature (30 degrees C) is beneficial to the shortening of analysis time. This method is fast, accurate and reproducible. It has good linear relationship in the range of 0.2-4 microg. The results have shown that the average recovery is 96.4% and coefficient of variation is 2.8%.
茄尼醇是一种主要从烟草中分离出来的萜类化合物，是维生素K2和辅酶Q10等药物的重要中间体。本文介绍了一种用高效液相色谱法测定烟叶提取物中茄尼醇含量的方法。采用内径为4.6 mm、长250 mm的Zorbax-SIL柱，以正己烷、异丙醚和乙酸乙酯（6:3:1）的混合液为流动相，可使茄尼醇得到完全分离，并用示差折光检测器进行测定。异丙醚可使色谱峰变尖锐，加入乙酸乙酯可防止分析过程中柱子堵塞，适宜的温度（30℃）有利于缩短分析时间。该方法快速、准确、重现性好。在0.2 - 4μg范围内线性关系良好。结果表明，平均回收率为96.4%，变异系数为2.8%。
