Zhang J, Shi W, Wang L, Zou H, Zhang Y
National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian, 116011.
Se Pu. 1997 May;15(3):197-200.
The influence of operation conditions, such as, injection time, applied voltage and effective length of capillary column, on parameters of peak shape in capillary zone electrophoresis (CZE) was investigated by using exponential-modified Gaussian (EMG) equation. The CE experiments were carried out by using the sample of derivatives of benzoic acid and small peptides. The experimens indicated that there was a significant effect of injection time on W1/2 and that the peaks changed from tailing to leading when the injection time was over 3 seconds in the case of pressure injection. It is recommended that an injection time less than 3 seconds can lead to a good column efficiency. The good linear relationships between tm and tau, sigma, W1/2 (t) with different separation voltages were observed. In the range of experimental conditions (11-27kV), higher voltage could lead to smaller zone dispersion, giving rapid and high efficient separation, and the voltage was insignificant on peak tailing. It was also shown that sigma, tau and W1/2 increased slightly with increasing the effective length of capillary column.
采用指数修正高斯（EMG）方程，研究了诸如进样时间、施加电压和毛细管柱有效长度等操作条件对毛细管区带电泳（CZE）中峰形参数的影响。使用苯甲酸衍生物和小肽样品进行了CE实验。实验表明，进样时间对半高峰宽有显著影响，在压力进样情况下，当进样时间超过3秒时，峰形从拖尾变为前伸。建议进样时间小于3秒可获得良好的柱效。观察到在不同分离电压下，迁移时间与弛豫时间、标准差、半高峰宽（t）之间具有良好的线性关系。在实验条件范围（11 - 27kV）内，较高电压可导致较小的区带展宽，实现快速高效分离，且电压对峰拖尾影响不显著。还表明，标准差、弛豫时间和半高峰宽随毛细管柱有效长度的增加而略有增加。
