Jin Y, Ban Y, Sun Y
Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang, 110015.
Se Pu. 1997 May;15(3):263-5.
Much effort in TLC has been devoted to optimization methods of solvent system in order to get best separation of components. Various optimization methods have been established, but the universality of some methods was poor. They were only applicable to samples of known components. This paper reports a complex method. When the complexity of the solvent system was determined, uniform design was used to arrange experiments, the data obtained from scanning chromatogram were input computer to get regression equation. The precision of the regression equation should be judged at first. If the precision was proper, the regression equation could be input the program of the complex method to obtain the composition of the solvent system. Optimization results were dichloroethane-benzene-methanol (4.81:4.42:0.78) for cortical hormone; toluene-ethanol-dichloroethane-trichloromethane (2.30:1.46:3.32:2.92) for sulfa drugs and trichloromethane-methanol-ethyl acetate (3.30:3.29:3.41) for ginkgo biloba. Then the verification experiment was made. The verifying test showed that the experimental results are identical with the calculated ones.
薄层色谱法（TLC）已投入大量精力用于优化溶剂系统方法，以便实现各组分的最佳分离。已建立了各种优化方法，但有些方法的通用性较差。它们仅适用于已知组分的样品。本文报道了一种综合方法。确定溶剂系统的复杂性时，采用均匀设计安排实验，将扫描色谱图获得的数据输入计算机以得到回归方程。首先应判断回归方程的精度。如果精度合适，可将回归方程输入综合方法程序以获得溶剂系统的组成。皮质激素的优化结果为二氯乙烷 - 苯 - 甲醇（4.81:4.42:0.78）；磺胺类药物的优化结果为甲苯 - 乙醇 - 二氯乙烷 - 三氯甲烷（2.30:1.46:3.32:2.92）；银杏叶的优化结果为三氯甲烷 - 甲醇 - 乙酸乙酯（3.30:3.29:3.41）。然后进行了验证实验。验证试验表明实验结果与计算结果一致。
