采用蛋白质工程化吡咯喹啉醌葡萄糖脱氢酶的新型葡萄糖酶燃料电池系统的开发。
Development of a novel glucose enzyme fuel cell system employing protein engineered PQQ glucose dehydrogenase.
作者信息
Yuhashi Noriko, Tomiyama Masamitsu, Okuda Junko, Igarashi Satoshi, Ikebukuro Kazunori, Sode Koji
机构信息
Department of Life Science and Biotechnology, Tokyo University of Agriculture and Technology, Nakamachi 2-24-16, Koganei, Tokyo 184-8588, Japan.
出版信息
Biosens Bioelectron. 2005 Apr 15;20(10):2145-50. doi: 10.1016/j.bios.2004.08.017.
Glucose dehydrogenase harboring pyrroloquinoline quinone as the prosthetic group (PQQGDH) from Acinetobacter calcoaceticus is an ideal enzyme for the anode of biofuel cell, because of its oxygen insensitivity and high catalytic efficiency. However, the application of PQQGDH for the bioanode is inherently limited because of its instability. Using Ser415Cys mutant whose stability was greatly improved, we constructed the biofuel cell system employing the engineered PQQGDH as the bioanode enzyme and bilirubin oxidase (BOD) as the biocathode, and compared the stability of the biofuel cell with that employing wild-type PQQGDH. The maximum power density was 17.6 microW/cm2 at an external optimal load of 200 k omega. Using Ser415Cys mutant, the lifetime of the biofuel cell system was greatly extended to 152 h, more than six times as that of the biofuel cell employing the wild-type.
来自醋酸钙不动杆菌的以吡咯喹啉醌为辅基的葡萄糖脱氢酶（PQQGDH）是生物燃料电池阳极的理想酶，因为它对氧气不敏感且催化效率高。然而，由于其不稳定性，PQQGDH在生物阳极上的应用受到固有限制。我们使用稳定性大大提高的Ser415Cys突变体，构建了以工程化PQQGDH作为生物阳极酶和胆红素氧化酶（BOD）作为生物阴极的生物燃料电池系统，并将该生物燃料电池的稳定性与使用野生型PQQGDH的生物燃料电池进行了比较。在200 kΩ的外部最佳负载下，最大功率密度为17.6微瓦/平方厘米。使用Ser415Cys突变体，生物燃料电池系统的寿命大大延长至152小时，是使用野生型的生物燃料电池寿命的六倍多。
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