相变影响磷脂囊泡中的能量转移效率。

Phase transition affects energy transfer efficiency in phospholipid vesicles.

作者信息

Kozyra K A, Heldt J R, Engelke M, Diehl H A

机构信息

Institute of Experimental Physics, University of Gdansk, ul.Wita Stwosza 57, Gdansk, Poland.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2005 Apr;61(6):1153-61. doi: 10.1016/j.saa.2004.06.034.

DOI:10.1016/j.saa.2004.06.034

PMID:15741115

Abstract

The fluorescence quenching of 6-propionyl-2-dimethylaminonaphtalene (PRODAN) and 6-dodecanoyl-2-dimethylaminonaphtalene (LAURDAN) by octadecyl rhodamine B (ORB) in a model system of small unilamellar vesicles (SUV) of dipalmitoylphosphatidyl-choline (DPPC) was investigated. Non-linear Stern-Volmer behaviour was observed in both systems in the gel phase (25 degrees C) and in the fluid phase (50 degrees C), resulting from association processes and from static quenching. The relative quenching efficiencies of both dyes depend on the phase state of the bilayer and indicate a deeper incorporation of PRODAN and LAURDAN into the membrane in its fluid phase than in its gel phase.

摘要

研究了在二棕榈酰磷脂酰胆碱（DPPC）小单层囊泡（SUV）模型体系中，十八烷基罗丹明B（ORB）对6-丙酰基-2-二甲基氨基萘（PRODAN）和6-十二烷酰基-2-二甲基氨基萘（LAURDAN）的荧光猝灭作用。在凝胶相（25℃）和流体相（50℃）的两个体系中均观察到非线性斯特恩-沃尔默行为，这是由缔合过程和静态猝灭导致的。两种染料的相对猝灭效率取决于双层膜的相态，表明PRODAN和LAURDAN在流体相的膜中比在凝胶相的膜中掺入得更深。