Kozyra K A, Heldt J R, Heldt J
Institute of Experimental Physics, University of Gdańsk, Wita Stwosza 57, 80-952 Gdańsk, Poland.
Biophys Chem. 2006 Apr 20;121(1):57-64. doi: 10.1016/j.bpc.2005.11.009. Epub 2006 Jan 27.
Fluorescence quenching and resonance energy transfer have been studied by steady-state fluorescence spectroscopy. The experimental and theoretical values for the rate constants of the electronic energy transfer (kET) and critical radius (R0) were determined for prodan and laurdan as donors and octadecyl rhodamine B as acceptor. The spectroscopic data show, that prodan and laurdan in solution create an inhomogeneous spectroscopic medium in which multi-channel luminescence phenomena take place. This finding indicated that the modified form of the Stern-Volmer relation should be used for analyzing fluorescence quenching data. Results of performed studies point out, that dipole-dipole interaction is responsible for the resonance energy transfer from prodan and laurdan to octadecyl rhodamine B. The relative quenching efficiencies of both dyes depend on polarity of the medium and are higher for more polar solvent (AcN).
通过稳态荧光光谱法研究了荧光猝灭和共振能量转移。以丙丹和月桂丹为供体,十八烷基罗丹明B为受体,测定了电子能量转移速率常数(kET)和临界半径(R0)的实验值和理论值。光谱数据表明,溶液中的丙丹和月桂丹形成了一种非均匀光谱介质,其中发生多通道发光现象。这一发现表明,应使用修正形式的斯特恩-沃尔默关系来分析荧光猝灭数据。所进行研究的结果指出,偶极-偶极相互作用是丙丹和月桂丹向十八烷基罗丹明B发生共振能量转移的原因。两种染料的相对猝灭效率取决于介质的极性,在极性更强的溶剂(乙腈)中更高。