Mannick E E, Schurr J R, Zapata A, Lentz J J, Gastanaduy M, Cote R L, Delgado A, Correa P, Correa H
Louisiana State University, Stanley S Scott Cancer and Depts of Pathology and Genetics, New Orlands, Louisiana 70112, USA.
Scand J Gastroenterol. 2004 Dec;39(12):1192-200. doi: 10.1080/00365520410003588.
Helicobacter pylori infection has protean effects on gene expression in the host gastric mucosa, which have been investigated by gene chip analysis in vitro. In this study the effects of H. pylori infection on host gene expression in the gastric antral mucosa in patients were examined.
One gastric antral biopsy was obtained from a total of 18 untreated patients undergoing routine endoscopic evaluation of chronic abdominal complaints. Nine patients had histologic evidence of H. pylori infection and 9 age- and sex-matched patients had no histologic evidence of H. pylori infection. A microarray analysis was performed using a gene chip containing 35,000 human expressed sequence tags on RNA extracted from endoscopic, gastric antral biopsies, and average gene expression among infected and uninfected patients was compared.
Underexpressed genes in infected patients' mucosa included gastric intrinsic factor and several metallothionein isoforms. Overexpressed genes in infected patients' mucosa comprised MHC Class II molecules, immunoglobulin and B-cell activation genes, as well as genes known to induce apoptosis. Changes in expression were confirmed for a subset of genes by SYBR green real-time PCR.
Microarray analysis of antral biopsies from patients with and without H. pylori infection revealed differential expression of metal regulatory, immunity and inflammation-related genes.
幽门螺杆菌感染对宿主胃黏膜中的基因表达具有多种影响，体外基因芯片分析已对这些影响进行了研究。在本研究中，检测了幽门螺杆菌感染对患者胃窦黏膜中宿主基因表达的影响。
从总共18例因慢性腹部不适接受常规内镜检查的未治疗患者中获取一份胃窦活检组织。9例患者有幽门螺杆菌感染的组织学证据，9例年龄和性别匹配的患者无幽门螺杆菌感染的组织学证据。使用包含35,000个人类表达序列标签的基因芯片对从内镜下胃窦活检组织中提取的RNA进行微阵列分析，并比较感染患者和未感染患者之间的平均基因表达。
感染患者黏膜中表达下调的基因包括胃内因子和几种金属硫蛋白异构体。感染患者黏膜中表达上调的基因包括MHC II类分子、免疫球蛋白和B细胞活化基因，以及已知可诱导细胞凋亡的基因。通过SYBR green实时PCR对一部分基因的表达变化进行了确认。
对有和没有幽门螺杆菌感染的患者的胃窦活检组织进行微阵列分析，揭示了金属调节、免疫和炎症相关基因的差异表达。
