Krejcírová Lenka, Lauschová Irena, Horký Drahomír, Doubek Michal, Mayer Jirí, Doubek Jirí
Department of Histology and Embryology, Medicine Faculty Masaryk University Brno, Czech Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2004 Dec;148(2):221-3. doi: 10.5507/bp.2004.044.
Amphotericin B deoxycholate (AmB) or Amphotericin B colloidal dispersion (ABCD) are used in clinics for the treatment of systemic fungal infections. The goal of our study was to compare the nephrotoxicity of these drugs in rat kidney. The effects of AmB and ABCD on the ultrastructure of the epithelium of renal tubules were studied and evaluated using morphometric and statistical methods. Two groups of 3 animals were established: group 1 was treated with AmB desoxycholate and group 2, to which ABCD was applied. AmB caused more than ABCD ultrastructural changes in the cytoplasm of the epithelial cells: damage to mitochondria, vacuolation of cytoplasm, and increased values of volume density of peroxisomes. However, we failed to observe significant differences in morphology and density of the other cell organelles. The proximal tubules seemed to be more sensitive to the nephrotoxic influence of both formulas than the distal tubules of rat kidney. Although, AmB causes more severe damage than ABCD, both drugs cause damage to renal tubuli.
两性霉素B去氧胆酸盐（AmB）或两性霉素B胶体分散体（ABCD）在临床上用于治疗全身性真菌感染。我们研究的目的是比较这些药物对大鼠肾脏的肾毒性。使用形态计量学和统计学方法研究并评估了AmB和ABCD对肾小管上皮超微结构的影响。建立了两组，每组3只动物：第1组用两性霉素B去氧胆酸盐治疗，第2组应用ABCD。AmB比ABCD引起上皮细胞胞质更多的超微结构变化：线粒体损伤、细胞质空泡化以及过氧化物酶体体积密度值增加。然而，我们未观察到其他细胞器在形态和密度上有显著差异。大鼠肾脏的近端小管似乎比远端小管对两种配方的肾毒性影响更敏感。虽然，AmB比ABCD造成的损伤更严重，但两种药物都会对肾小管造成损伤。
