Halter S A, Webb L, Rose J
Department of Pathology, Vanderbilt University, Nashville, Tennessee.
Mod Pathol. 1992 Mar;5(2):131-4.
Ras oncogene mutations are found in a significant number of human colonic carcinomas. Correlation between patient survival and ras mutations was explored and compared with other clinical parameters. Colonic carcinoma embedded in paraffin was subjected to the polymerase chain reaction using primers to amplify codon 12 of the K-ras oncogene. Oligonucleotide probes to six mutations were used to identify mutated genes. A total of 71 cases were successfully amplified. Some 54% of the cases had mutations. There was no correlation between presence of a mutation and age. Patients in Stage D, patients with a family history of carcinoma, and males have a greater incidence of ras mutations. Patients in Stage C had a lower incidence of mutations. Presence of the mutation did not correlate with decreased survival except in Stage D. Some 61% of long-term survivors with colon carcinoma living for more than 6 yr had ras mutations. The absence of K-ras mutations did not identify long-term survivors.
在大量人类结肠癌中发现了Ras癌基因突变。研究了患者生存率与ras突变之间的相关性，并与其他临床参数进行了比较。使用针对K-ras癌基因第12密码子的引物，对石蜡包埋的结肠癌进行聚合酶链反应。使用针对六种突变的寡核苷酸探针来鉴定突变基因。总共71例成功扩增。约54%的病例有突变。突变的存在与年龄无关。D期患者、有癌家族史的患者和男性的ras突变发生率更高。C期患者的突变发生率较低。除了D期，突变的存在与生存率降低无关。约61%存活超过6年的结肠癌长期幸存者有ras突变。K-ras突变的缺失并不能识别长期幸存者。
