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蛋白质浓度、pH值、乳糖含量和巴氏杀菌对酸法山羊乳清浓缩蛋白热凝胶化的影响。

Effect of protein concentration, pH, lactose content and pasteurization on thermal gelation of acid caprine whey protein concentrates.

作者信息

Bordenave-Juchereau Stéphanie, Almeida Bruno, Piot Jean-Marie, Sannier Frédéric

机构信息

Laboratoire de Biotechnologies et de Chimie Bio-organique, CNRS FRE 2766, UFR Sciences, Batiment Marie Curie, Avenue Michel Crepeau, La Rochelle, France.

出版信息

J Dairy Res. 2005 Feb;72(1):34-8. doi: 10.1017/s0022029904000482.

DOI:10.1017/s0022029904000482
PMID:15747729
Abstract

The influence of pH (4.5-6.5), sodium chloride content (125-375 mM), calcium chloride content (10-30 mM), protein concentration (70-90 g/l) and lactose content on the gel hardness of goat whey protein concentrate (GWPC) in relation to the origin of the acid whey (raw or pasteurized milk) was studied using a factorial design. Gels were obtained after heat treatment (90 degrees C, 30 min). Gel hardness was measured using texture analyser. Only protein concentration and pH were found to have a statistically significant effect on the gel hardness. An increase in the protein concentration resulted in an increase in the gel hardness. GWPC containing 800g/kg protein formed gels with a hardness maximum at the pHi, whereas GWPC containing 300 g/kg protein did not form true gels. Whey from pasteurized milk formed softer gels than whey from raw milk. A high lactose content (approximately 360 g/kg) also reduced the gelation performance of GWPC.

摘要

采用析因设计研究了pH值(4.5 - 6.5)、氯化钠含量(125 - 375 mM)、氯化钙含量(10 - 30 mM)、蛋白质浓度(70 - 90 g/l)和乳糖含量对山羊乳清浓缩蛋白(GWPC)凝胶硬度的影响,该影响与酸乳清的来源(生乳或巴氏杀菌乳)有关。凝胶在热处理(90℃,30分钟)后获得。使用质构分析仪测量凝胶硬度。结果发现,只有蛋白质浓度和pH值对凝胶硬度有统计学上的显著影响。蛋白质浓度的增加导致凝胶硬度增加。含有800g/kg蛋白质的GWPC在pH值为i时形成硬度最大的凝胶,而含有300 g/kg蛋白质的GWPC则未形成真正的凝胶。巴氏杀菌乳的乳清形成的凝胶比生乳的乳清形成的凝胶更软。高乳糖含量(约360 g/kg)也会降低GWPC的凝胶性能。

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