源自间充质干细胞的成骨细胞具有免疫调节作用。

[Osteoblasts derived from mesenchymal stem cells harbor immunoregulatory effect].

作者信息

Jiang Xiao-Xia, Zhang Yi, Li Xiu-Sen, Wu Ying, Yu Xiao-Dan, Tang Pei-Hsien, Mao Ning

机构信息

Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Feb;13(1):50-3.

PMID:15748435

Abstract

In an attempt to study the immunoregulatory effect of osteoblasts derived from mesenchymal stem cells (MSC), MSC was induced to differentiate into osteoblasts for one week. The growth pattern and the phenotype were evaluated by MTT and flow cytometry respectively. The immunoregulatory effect was tested by the inhibitory effect on T cell proliferation. The result showed that during the differentiation cells grew fast and there was no significant change in the phenotypes but keeping CD73, CD105, CD44, CD29 positive and CD34, CD45, HLA-DR, CD86 negative. Osteocyte derived from MSC also showed immunosuppressive effect on T cell proliferation in adose-dependent manner. It is concluded that osteoblasts derived from MSC also harbored immunoregulatory effect.

摘要

为了研究间充质干细胞（MSC）来源的成骨细胞的免疫调节作用，将MSC诱导分化为成骨细胞一周。分别通过MTT和流式细胞术评估生长模式和表型。通过对T细胞增殖的抑制作用来测试免疫调节效果。结果显示，在分化过程中细胞生长迅速，表型无明显变化，但保持CD73、CD105、CD44、CD29阳性，CD34、CD45、HLA-DR、CD86阴性。MSC来源的骨细胞对T细胞增殖也呈剂量依赖性的免疫抑制作用。结论是，MSC来源的成骨细胞也具有免疫调节作用。