Wu Xiu-Li, Li Yang-Qiu, Wang Zhen, Yang Li-Jian, Chen Shao-Hua, Zhang Huan, Zhu Kang-Er, Han Zhong-Chao
Institute of Hematology, Medical College, Jinan University, Guangzhou 510632,China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Feb;13(1):70-5.
In order to investigate expressions of transcription factor GATA-1 and GATA-2 genes in the bone marrow stromal cells (BMSCs) from patients with leukemia or normal controls, bone marrow stromal cells from 34 normal cases and 42 cases with leukemia were cultured long-term in vitro. Nonadherent cells (bone marrow hematopoietic cells) and amplified adherent cells (BMSC) were collected separately. Expressions of GATA-1 and GATA-2 genes were analyzed by using RT-PCR-ELISA; the semi-quantitative expression levels of GATA genes in the BMSCs from patients with leukemia were compared with normal controls. The results showed that expressions of GATA-1 and GATA-2 genes could be detected in the BMSCs and the bone marrow hematopoietic cells from both normal controls and the cases of leukemia. The expression ratio of GATA-1 in the BMSCs from acute lymphocytic leukemia (ALL) (85.7%) was similar to the normal controls (88.2%), whereas the expression ratios in BMSCs from acute myelocytic leukemia (AML) (55.6%) and chronic myelocytic leukemia (CML) (41.2%) were significant lower than the normal controls (P < 0.05). The rank of expression level of GATA-1 gene in the BMSCs was "ALL>AML>normal>CML". There was no difference in the expression level of GATA-2 gene within the BMSCs from normal controls and patients with leukemia. The ranks of expression levels of GATA-1 and GATA-2 genes in bone marrow hematopoietic cells were "AML>normal>ALL>CML" and "AML>CML>ALL>normal". The dominant expression of GATA-2 gene was found in the BMSCs from AML, CML or normal controls. It is inferred that the expressions of GATA-1 and GATA-2 genes in the BMSCs of normal controls and patients with leukemia may influence the regulation of hematopoiesis in the bone marrow stroma and it is worthy of further study to explore their roles in pathogenesis and development of leukemia.
为研究白血病患者与正常对照者骨髓基质细胞（BMSCs）中转录因子GATA-1和GATA-2基因的表达情况，对34例正常人和42例白血病患者的骨髓基质细胞进行长期体外培养。分别收集非贴壁细胞（骨髓造血细胞）和扩增后的贴壁细胞（BMSC）。采用RT-PCR-ELISA法分析GATA-1和GATA-2基因的表达；比较白血病患者BMSCs中GATA基因的半定量表达水平与正常对照者的差异。结果显示，正常对照者和白血病患者的BMSCs及骨髓造血细胞中均可检测到GATA-1和GATA-2基因的表达。急性淋巴细胞白血病（ALL）患者BMSCs中GATA-1的表达率（85.7%）与正常对照者（88.
