Domènech Oscar, Torrent-Burgués Juan, Merino Sandra, Sanz Fausto, Montero M Teresa, Hernández-Borrell Jordi
Departament de Química Física, Universitat de Barcelona, Centre de Bioelectrònica i Nanobiociència (CBEN), Parc Científic de Barcelona, Josep Samitier 1-5, E-08028 Barcelona, Spain.
Colloids Surf B Biointerfaces. 2005 Apr 10;41(4):233-8. doi: 10.1016/j.colsurfb.2004.12.012.
The interaction of 1-palmitoy-2-oleoyl-sn-glycero-3-phosphocoline (POPC) and 1-palmitoy-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), two of the major components in biological membranes, were investigated using the monolayer technique at the air-water interface. The pressure-area isotherms indicate that both phospholipids are miscible through all range of compositions. POPE-POPC form stable mixtures, with a minimum for the Gibbs energy of mixing at X(POPC) = 0.4. A virial equation of state was fitted to the experimental values. Positive values found for the second virial coefficient indicate repulsion between POPC and POPE. The interaction parameter was evaluated which indicated that a corresponding decrease in the repulsion occurs when POPC molar fraction is low. This effect suggests the existence of hydrogen bonds between POPE and the water beneath the interface.
利用空气-水界面的单分子层技术，研究了生物膜中两种主要成分1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱（POPC）和1-棕榈酰-2-油酰-sn-甘油-3-磷酸乙醇胺（POPE）之间的相互作用。压力-面积等温线表明，两种磷脂在所有组成范围内都是可混溶的。POPE-POPC形成稳定的混合物，在X（POPC）=0.4时混合吉布斯自由能最小。将维里状态方程拟合到实验值。第二维里系数的正值表明POPC和POPE之间存在排斥作用。评估了相互作用参数，结果表明当POPC摩尔分数较低时，排斥作用相应降低。这种效应表明POPE与界面下方的水之间存在氢键。
